Calcein blue

Buryak and Severin have described the use of dynamic libraries of Cu(II) and Ni(II) complexes as sensors for tripeptides [61]. A notable aspect of this work is that as isolation of the metal complexes is not necessary (sensing is accomplished by observing changes in the UV-vis spectrum), potential concerns over the lability of coordination complexes do not apply. Specifically, three common dyes [Arsenazo I (41), Methyl Calcein Blue (42), and Glycine Cresol Red (43), Fig. 1.18] were mixed with varying ratios and total concentrations of Cu(II) and Ni(II) salts in a 4X5 array. Previous work had demonstrated that these conditions produced equilibrating mixtures of 1 1 and 2 1 homo- and heteroleptic complexes [62], These arrays were able to clearly and unambiguously differentiate tripeptides based on the differential pattern of response. The Severin laboratory has... 

The first such sensor, developed for the determination of fluoride ion, relies on the formation of the zirconium(IV)-Calcein Blue-fluoride complex... 

By introducing the complexing moiety —CH2N(CH2C02H)2 into fluorescein or umbelliferone metallochromic indicators termed calcein or fluorexone, and calcein blue or umbellicomplexone are formed. These indicators exhibit green (and blue) fluorescence in dilute alkali which disappears at pH 12 but reappears on the addition of calcium, strontium, or barium. Problems arise from the difficulty of freeing the indicators from fluorescent impurities, from self-fluorescence, and from the quenching effect of copper ions. 

Yokoi, K., Yamaguchi, A., Mizumachi, M. and Koide, T. (1995) Direct determination of trace concentrations of lead in fresh water samples by adsorptive cathodic stripping voltammetry of a lead-Calcein Blue complex. Anal. Chim. Acta, 316, 363-369. 

Chen et al.[58] described a sensitive and selective fluorimetric method for the determination of fluoride by measuring the fluorescence of the ternary complex of zirconium, Calcein Blue and fluoride retained on DEAE-Sephadex anion-exchanger in a 1.1 mm i.d. flow-cell. The detection limit for fluoride was 1 /g 1 at a sampling frequency of 30 h and consuming only 0.5 ml of sample. An excellent precision of 1% r.s.d. was reported, and the tolerance to most interfering ions has been improved substantially, some by an order of magnitude, compared to a manual method based on the same reaction. 

Leong, W. L., Vittal. J. J. (2009). Synthesis and characterization of metal complexes of Calcein Blue Formation of monomeric, ion pair and coordination polymeric structures. Inorg. Chem. Acta, 362, pp. 2189-2199, ISSN 0020-1693... 

Bis(carboxymethyl)amlnomethyl-7-hydroxy-4-methyl-l,2-benzopyranone 74HP (Calcein Blue) (C gH O N)... 

Figure 15 (a) Generation of a DCL of metal-dye complexes by mixing arsenazo I (35), methyl calcein blue (36), and glycine cresol... 

Wilkins, D. H. Calcein Blue—a new metal fluorochromic indicator for chelatometric titrations. Talanta 1960, 4, 182-184. 

Goto, T. Kajiwara, H. Yoshinari, M. Fukuhara, E. Kobayashi, S. Tanaka, T. In vitro assay of mineralized-tissue formation on titanium using fluorescent staining with calcein blue. Biomaterials 2003, 24, 3885-3892. 

Gholivand, M. B. Sohrabi, A. Abbasi, S. Determination of copper by adsorptive stripping voltammetry in the presence of calcein blue. Electroanalysis 2007, 19, 1609-1615. 

Matsoukas, K. A. Demertzis, M. A. Fluorometric deter-mination of calcium in serum with calcein blue. Analyst 1988, 113, 251-253. 

Yokoi, K. Mizumachi, M. Koide, T. Determination of cadmium by adsorptive stripping voltammetry of a cadmium-calcein blue complex. Anal. Sci. 1995,11, 257-260. 

Brittain, H. G. Submicrogram determination of lanthanides through quenching of calcein blue fluorescence. Anal. Chem. 1987, 59, 1122-1125. 

Escarrilla, A. M. Calcein blue as a fluorescent adsorption indicator in the direct titration of silver ions. Anal Chim. Acta 1968, 43, 353-356. 

Zou, S. Aodeng, G. Sai, Y. A new spectrophotometric fluorescence method for the determination of trace zinc (II) in foodstuff using calcein blue. Fenxi Shiyanshi 1995, 14, 45-48 Chem. Abstr. 1996, 124, 305850. 

Ishibashi, N. Imasaka, T. Ogawa, T. Maeda, M. Miyazoe, Y. Coordination compounds of calcein blue for new laser material. Chem. Lett. 1974, 3i5-1316. 

CA Index Name Glycine, A-[2-[(acetyloxy)methoxy]-2-oxoethyl]-A-[(7-hydroxy-4-methyl-2-oxo-2//-1 -benzo-pyran-8-yl)methyl]-, (acetyloxy)methyl ester Other Names Calcein Blue AM Calcein Blue acetoxy-methyl ester... 

Calcein Blue Calcium Cadmium 78 Fura 2 AM Calcium 215... 

Other Names Calcein Blue AM, Calcein Blue acetoxymethyl ester CellTrace Calcein Blue AM Merck Index Number Not listed Chemical/Dye Class Coumarin... 

Bromomethyl-6,7-dime thoxy coumarin 4-Bromomethyl-7-methoxycoumarin Calcein Blue AM... 

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