C Antigenicity

Brown, C. Antigen retrieval methods for immunohistochemistry. Toxicol. Pathol. 

A 74-year-old woman who had used acarbose for 3 months developed progressive weakness and jaundice (60). Her bilirubin was 152 pmol/l (direct bilirubin 96 pmol/1). All of her liver enzymes were substantially raised. All other investigations were normal, except that she was positive for hepatitis C antigen. After withdrawal of acarbose everything became normal within 1 month. 

Eriksson K, Ahlfors E, George-Chandy A, Kaiserlian D, Czerkinsky C Antigen presentation in the murine oral epithelium. Immunology 1996 88 147-152. 

Shokat, K. M., and Goodnow, C. C., Antigen-induced B-cell death and elimination during germinal-centre immune responses. Nature 375, 334-338 (1995). 

T. Bartui, G. Flnazzo, S. Vipio, and P. M. Manued. 1-Asparaginase lowers protein C antigen. Thromb. Haemost 52 216 (1984),... 

Vigano S, Mannucci PM, Solinas S, et al. Decrease in protein C antigen and formation of an abnormal protein soon after starting oral anticoagulant therapy. Br J Haematol 1984 57(2) 213-20. 

Brody RI, Eng S, Melamed J, et al. Immunohistochemical detection of hepatitis C antigen by monoclonal antibody TORDJl-22 compared with PCR viral detection. Am J Clin Pathol. 1998 110 32-37. 

Deoxy-L-talose (20) sarmentoside A and related substances acolongifloroside K 80 174, 176 181 Strophanthus thoUonii Strophanthus sarmentosiis Acokanthera longifiora and others Actinomyces bovis Mycobact. avium Pseudomonas pseudomallei cell walls after hydrolysis Mycoside C antigen K after hydrolysis 182, 183 182, 184,185 182... 

An alternative explanation is that inactivation of picornaviruses reflects a concerted conformational rearrangement of the capsid proteins, and the loss of 7P4 (isoelectric focusing studies of poliovirus and a human rhinovirus. Poliovirions were resolved into two interconvertible foims with isoelectric pH s of 4 5 and 7 0> "the latter form being infectious. Treatments which inactivated the virus (attachment and elution from cells or UY irradiation) produced only the non-infectious acidic form (49)- Similarly, when a purified human rhinovirus preparation was subjected to isoelectric focusing in a sucrose gradient two forms of the virion were separated (a) isoelectric pH = 6.5> 150 .9 infectious, D antigenicity and (b) isoelectric pH =4.5, 140, non-infectious, indirect evidence for C antigenicity. 

Both forms, however, contained intact RNA and the full complement of capsid polypeptides (50) Further evidence that a substantial conformational alteration of entero- and rhinovirus capsids accompanies the D to C antigenicity change has been obtained by examining the accessibilities of individual capsid proteins in intact virions, A-particles and naturally-occurring empty capsids to lacto-peroxidase-catalyzed iodination or alkylation with acetic anhydride (5I> 52). The results of these experiments are summarized in Table lY. Since under the experimental conditions employed the radioactive reagents formed covalent bonds with tyrosine residues... 

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