1,4-Butanediol, glycerol dehydrogenase

Fig. 9 Stereoselective oxidation of 1,2-butanediol to (S)-1,2-butanediol catalyzed by glycerol dehydrogenase with simultaneous cofactor regeneration using the system 2-ketoglutarate/gluta-mate dehydrogenase (GluDH)...

Resting cells of a recombinant K. pneumoniae J2B strain that overexpresses an aldehyde dehydrogenase (KGSADH) were utilized for coproduction of 3-HP and 1,3-pro-panediol (PDO) from glycerol. The fed-batch bioconversion was performed under anaerobic conditions in a 1.5 L bioreactor with l.Og CDW/L at a constant pH of 7.0 and resulted in 125.6mM 3-HP and 209.5 mM PDO after 12 h with a cumulative overall productivity, yield, and maximum specific production rate of 27.9mmol/Lh, 0.71 (mol/mol), and 128.5mmol/g CDW/h, respectively.The major by-products were lactate, succinate, and 2,3-butanediol. °... 

Palosaari NR, Rogers P (1988) Purification and properties of the indudble coenzyme A-linked butyraldehyde dehydrogenase from Clostridium acetobutylicum. J Bacteriol 170 2971-2976 Papanikolaou S, Ruiz-Sanchez P, Pariset B, Blanchard F, Fick M (2000) High production of 1,3-propanediol from industrial glycerol by a newly isolated Clostridium butyricum strain. J Biotechnol 77 191-208 Papoutsakis ET, Meyer CL (1985) Equations and calculations of product yields and preferred pathways for butanediol and mixed-acid fermentations. Biotechnol Bioengin 27 50-66... 

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