Buspirone, determination

FIGURE 10.2 Metabolite profiles of buspirone determined by HPLC with MSC, LSC, and RFD (Zhu et al., 2005b). Buspirone metabolites from human liver microsome incubations were separated by HPLC (1 mL/min). Radioactivity in HPLC effluent was determined by (a) TopCount 8000 DPM injected, four fractions per min and 10 min counting time (b) LSC 8000 DPM injected, two fractions per min, 10 min counting time and (c) RFD 32,000 DPM injected. 

Kivisto KT, Laitila J, Martensson K, Neuvonen PJ. 1999. Determination of buspirone and l-(2-pyrimidinyl)-pipera-zine (1-PP) in human plasma by capillary gas chromatography. Ther Drug Monit 21 317. 

Lai C-T, Tanay VA-MI, Rauw GA, Bateson AN, Martin IL, et al. 1997. Rapid, sensitive procedure to determine Buspirone levels in rat brains using gas chromatography with nitrogen-phosphorus detection. J Chromatogr B 704 175. 

Ary K, Rona K, Ondi S, Gachalyi B. 1998. High-performance liquid chromatographic method with coulometric detection for the determination of buspirone in human plasma by means of a column-switching technique. J Chromatogr A 797(1-2) 221-226. 

Bianchi G, Caccia S. 1988. Simultaneous determination of buspirone, gepirone, ipsapirone and their common metabolite l-(2-pyrimidinyl)piperazine in rat plasma and brain by high-performance liquid chromatography. J Chromatogr 431(2) 477-480. 

First-line GAD treatments include (1) cognitive-behavioral therapy (CBT), (2) antidepressants, (3) buspirone, and (4) benzodiazepines. Treatment selection is determined by the severity of the illness, the presence of any comorbid illnesses, previous patient treatment responses, and patient preference. When treating mild GAD, we recommend eschewing psychotropic medication altogether in favor of CBT. Moderate-to-severe GAD usually requires pharmacotherapy, though combined CBT-pharmacotherapy is highly encouraged. 

Nagele, E. and Fandino, A.S., Simultaneous determination of metabolic stability and identification of buspirone metabolites using multiple column fast liquid chromatography time-of-flight mass spectrometry, J. Chromatogr. A, 1156(1-2), 196, 2007. 

Kristjansson, F. Sensitive determination of buspirone in serum by solid-phase extraction and two-dimensional high-performance liquid chromatography. J.Chromatogr., 1991, 566, 250—256... 

Franklin, M. Determination of plasma buspirone by high-performance liquid chromatography with cou-lometric detection. J.Chronuitogr., 1990, 526, 590-596... 

Diaz-Marot, A. Puigdellivol, E. Salvatella, C. Cornelias, L. Gassiot, M. Determination of buspirone and l-(2-pyrimidinyl)piperazine in plasma samples by high-performance liquid chromatography. 

Profiles of buspirone metabolites were determined by HPLC with three radiodetection techniques (see Fig. 10.2). Percent distribution of radioactive peaks was calculated by dividing the radioactivity of a metabolite peak by the total radioactivity determined in the HPLC run. ( 8000 DPM per injection for HPLC-LSC and HPLC-MSC, and 32,000 DPM per an injection for HPLC-RCD). 

Many drugs, such as buspirone, undergo multiple oxidative biotransformation reactions. In cases such as buspirone the secondary metabolites, which are minor metabolites in vitro, are major metabolites in the circulation or excreta in humans and animals because primary metabolites are rapidly converted to secondary metabolites. To determine the formation pathways and structures of secondary or sequential metabolites, a method using metabolite incubation and HPLC MSC MS analysis was developed and demonstrated using buspirone as an example (Zhu, 2002). [ Cjbuspirone was incubated with HLM, and metabolic profiling was carried out using HPLC-MSC. The... 

Zhu M, Zhang H, Zhao W, Mitroka J, Klunk L. A novel in vitro approach, using a microplate scintillation counter and LC/MS, for determining formation pathways and structures of sequential metabolites Buspirone as an example. Drug Metab Rev 2002 34 167. 

Sarati, S. Guiso, G. Spinelli, R. Caccia, S. Determination of piribedil and its basic metabolites in plasma by high-performance liquid chromatography. J.Chromatogr, 1991, 663, 323-332 [plasma rat extracted piribedil buspirone is IS gradient]... 

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