Bulk transfer of a liquid under inert atmosphere cannulation

2 Bulk transfer of a liquid under inert atmosphere (cannulation) 

As a general rule, never attempt to remove liquid from a container which is sealed under inert gas, unless you have pressurized the container with inert gas first (Fig. 6.2a). Also, whenever you are using ground glass joints connected under pressure always secure them with either plastic (Bibby-type) clips, elastic bands or springs. 

It is quite common that the flask into which you need to cannulate the liquid is already part of an inert gas system and can not simply be vented. In this case the pressure applied to the first flask must be higher than that in the receiving flask and there must be some means by which gas can escape from the receiving flask. The inert gas system to which the receiving flask is connected should incorporate a bubbler (for example, see Fig. 3.7) and 

The following procedure is a general method for transferring liquids by cannulation and will be referred to in other sections of the book. 

Make sure the bottle or flask into which you are going to transfer the reagent is thoroughly dry, fit a septum into the neck and purge with inert gas as described in the previous section. If the container was oven dried it is preferable to fit the septum whilst it is still hot and allow it to cool as it is being purged with inert gas (Fig. 6.1a). 

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