Buffers mixed

For maintenanee of optimum values pH at definition of Rh and Ir used the aeetie buffer mixes, prepared with addition of isopropanole. Colour of eomplexes develops within 2 h at 65 5°C or during 5 min under influenee of mierowave radiation. 

Aqueous perchlorate-citrate buffer mixed with CH3CN Sil-X, 13 ym, 5 x 125 mm 1.3 60... 

Ion-pair chromatography separates ionic compounds using traditional RP stationary phases. A so-called counter-ion of opposite charge is added to the mobile phase. It forms a neutral ion-pair which can be easily separated under RP conditions. The mobile phase generally consists of water or buffer mixed with an organic modifier such as methanol or ACN. 

Salicylic Acid Absorption. The apical 5 cm of the primary and two seminal roots from each of three plants were out into 1-cm segments to form an experimental unit (ca. 0.08 g). Incubation solution cc ijjtalned 0.5 mM KCl, 0.25 mM CaSOjj, 0.5 mM salicylic acid, 10 nCl/mL [ C]-sallcyllc acid, with 25 mM Tris and 25 mM Mes buffers mixed to obtain pH 6.5. Because the salicylic acid was dissolved in absolute ethanol, the final concentration of ethanol in the incubation solution was 1 (v/v). Root segments were transferred to test tubes containing 10 mL continuously aerated incubation solution. After the predetermined absorption time, segments were collected from the incubation solution by rapid filtration on Whatman No. 2 filter paper. 

In the stripper/condenser, the gas is cooled by counterflowing condensate from the condenser. The temperature of the SO2 rich gas that leaves the condenser is used to control the amount of cooling medium that must be sent to the condenser. Condensate from the stripper is returned to the buffer mix tank. The SO2 rich gas, containing at least 90% SO2 with the remainder being water, is ready for transport to a process unit. In the refinery, this normally will be the SRU or acid plant, where it will be converted to elemental sulfur or sulfuric acid. The SO2 can also be compressed and transported if necessary. Eigure 16.9 shows a typical Belco LABSORB System. 

Reaction buffer Mix 20 ml PIPES buffer with 50 ml NaCl solution and add another 30 ml demineralized water in a 250-ml flask. The pH is adjusted to 6.5 with 0.1 M HC1. Fill to the 250-ml mark with demineralized water. 

Reaction buffer mix 57 ml 0.1 M citric acid with 43 ml 0.2 M phosphate solution and dissolve 1.17 g NaCl (0.2 M) in the buffer. Adjust the pH to 4.3 with either citric acid or phosphate solution. 

Reaction buffer mix 46.4 ml citric acid with 53.6 ml phosphate solution. Adjust the pFl to 5.2 with either citric acid or phosphate solution. 

Reaction buffer mix 70 ml sodium acetate with 30 ml acetic acid and adjust the pH to 5.0 with either sodium acetate or acetic acid solution. 

For medium-sized gels, add 1 - 3 g research-grade agarose or another agarose to 100 ml 1 x TBE buffer. Mix well and heat to boiling in a microwave oven until fully... 

Just before use, add 66 pL NBT stock solution to 10 mL of alkaline phosphatase buffer, mix well, and add 33 pL of BCIP stock solution. 

Nucleotides/buffer mix 4 mMrATP, 4 mM rGTP, 4 mMrCTP m 400 mMTris-... 

One of the major technical problems that had to be overcome to integrate the POLYBED system with the steam reformer was the variation in tail gas flow and composition. Because of the cyclic nature of the process, tail gas is rejected by the POLYBED unit during blowdown and purge with significant flow and composition variations. The fluctuations would have made it impossible to use the tail gas for fuel and a sophisticated system was developed to balance tail gas heating value. This buffer/mixing tank system has proven to be very reliable in holding heat input variation to 1% (2). ... 

Test solution 2 (for Capsules labeled 40 mg) Immediately transfer 5 ml of the solution under test to a test tube containing 2 ml of 0.25 M sodium hydroxide and 5 ml of pH 6.8 Phosphate buffer. Mix well, and pass through a membrane filter having a 1.2-pm or finer porosity. Protect from light. 

In a test tube, add 170 pi of the NADH stock solution, 100 pi of the 30 mM pyruvate stock solution, the appropriate volume of enzyme and then make up the quantity to 3 ml with phosphate buffer. Mix slowly then measure the variation of absorbance at 340 nm for 2 min. Calculate from the data obtained the specific enzyme activity. 

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