Buffered nutrient broth

Values of A were determined by experiments where the cells were irradiated in the phosphate buffer and in various nutrient-broth solutions (Fig. 3a). 

Exposure of 4 mg/ml for 4 hr to washed bacterial cells in 0.1 M, pH 7.5 phosphate buffer at 10 times the cell concentration of an 18-hr nutrient broth culture. Residual activity determined by bioassay. 

Bacteria were grown in nutrient broth (Difco) at 37 for 18 hours. Test suspensions were diluted to approximately 5 X 10 cells per ml. in O.IM phosphate buffer... 

The yeast cells employed in this study were all grown in shake flasks in nutrient broth plus 1% dextrose for a period of 36-40 hours at room temperature. The cell suspension was centrifuged and washed 3 times with sodium-potassium phosphate buffer, pH 7.2. The washed suspension of cells was added to the carrier and agitated by shaking for 3 hours of contact time. 

A 24 hour nutrient broth culture of S. aureus was diluted with sterilised 0.3 mM phosphate buffer to give a final concentration of 1-2 x lO CPU / ml. 50 ml of the diluted bacterial culture was transferred to sterilised 250 nd glass jars to tiriiich 1.0 g of test fabric, which had been cut into approximately 1 cm pieces then autoclaved, was added. The tops were screwed onto the jars and they were incubated and shaken at 120 rpm at 37 °C for 1 hour or 24 hours. After incubation, serial dilutions were performed and were plated onto nutrient agar using the Miles Misra method. The plates were incubated for 24 hours at 37 °C and the number of CPU / g of fabric was calculated. The antimicrobial activity of the tested fabric samples was expressed as a log reduction in which the mean loglO density of bacteria for the jar containing the test samples after... 

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