Miles Misra method

Surface spread or surface drop (Miles Misra) methods (counting colonies on agar surface)... 

A 24 hour nutrient broth culture of S. aureus was diluted with sterilised 0.3 mM phosphate buffer to give a final concentration of 1-2 x lO CPU / ml. 50 ml of the diluted bacterial culture was transferred to sterilised 250 nd glass jars to tiriiich 1.0 g of test fabric, which had been cut into approximately 1 cm pieces then autoclaved, was added. The tops were screwed onto the jars and they were incubated and shaken at 120 rpm at 37 °C for 1 hour or 24 hours. After incubation, serial dilutions were performed and were plated onto nutrient agar using the Miles Misra method. The plates were incubated for 24 hours at 37 °C and the number of CPU / g of fabric was calculated. The antimicrobial activity of the tested fabric samples was expressed as a log reduction in which the mean loglO density of bacteria for the jar containing the test samples after... 

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