BufFalo rat liver

Grow Buffalo rat liver (BRL) cells to confluence in Ham s F12 or DMEM containing 5% FBS. 

ATCC BHK cells BME BPL BRL BSA BSS BUdr CHO cells CMF CMP, CDP, CTP American Type Culture Collection baby hamster kidney cells basal medium (Eagle) /J-propiolactone Buffalo rat liver bovine serum albumin balanced or buffered salt solution bromodeoxyuridine Chinese hamster ovary cells calcium- and magnesium-free BSS cytidine monophosphate, diphosphate, triphosphate... 

A wide variety of data firom many laboratories indicates that the liver is a major source of somatomedin peptides. This has been demonstrated directly in studies of isolated perfused livers (F3, K13, MIO, P9, S9, S2I, W6), fetal (D14, R5) and adult (B25, S13) liver in organ culture, rat liver cell lines (M5, M26, S27), and primary hepatocyte cultures (K14, S22, S28). Tliese studies are supported by the observations that partial hepatectomy (U3) or liver disease (S14, T5) results in low circulating somatomedin activity. It has not always been clear, however, which members of the somatomedin family were being assayed in some of these studies, due to the broad specificity of the assays used (see Section 5). For example, whereas it has been well established that the BRL (buffalo rat liver) cell line, and fetal rat liver in organ culture (R5), produce peptides of the MSA or IGF-II family (M5, M26), it is not known whether normal adult liver produces IGF-II. Indeed, production of SM-G/IGF-I by adult liver has been demonstrated specifically in only two studies. In these, Schwander et al. (S21) found that a S-labeled product from perfused liver could be immunoprecipitated with an IGF-I antiserum, and Scott et al. (S22) used a specific SM-C/IGF-I RIA to demonstrate production of the peptide by adult hepatocytes in primary culture. In both studies, the measured hepatic production rate was calculated to be sufficient to account fully for circulating SM-C/IGF-I levels. 

K.A., Karkare, S.B., Sachdev, R.K., Yuschenkoff, V.N., Birkett, N.C., Williams, L.R., Satyagal, V.B., Tung, W., Bosselman, R.A., Mendiaz, E.A. and Langley, K.E. (1990b) Identification, purification and biological characterization of haematopoietic stem cell factor from Buffalo rat liver-condtioned medium. Cell 63 195-201. 

JR Olson State University of New York (SUNY) at Buffalo, NY Develop mechanism-based molecular and biochemical markers of exposure, effect, and/or susceptibility specific to 2,3,7,8-TCDD in maternal and fetal rat liver, placenta, and lymphocytes... 

Reuber MD, Glover EL. 1967. Hyperplastic and early neoplastic lesions of the liver in buffalo strain rats of various ages given subcutaneous carbon tetrachloride. J Natl Cancer Inst 38 891-899. 

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