BrdU labeling index

Administration of hydroquinone (0.5% in the diet) for 20 weeks did not induce hyperplasia or papillomatous lesions in the forestomach in Syrian golden hamsters (Hirose etal., 1986). In male Fischer 344 rats, oral administration of hydroquinone for eight weeks (0.8% in the diet) did not induce hyperplasia or DNA synthesis, as measured by BrdU-labelling index in the forestomach epithelium. No cell proliferation, increased DNA synthesis or increase in pepsinogen-isoenzyme-1-altered neoplastic foci was observed in the pyloric mucosa (Shibata et al., 1990). 

BrdU labeling index (BrdU Li) Mammary gland (BrdU Li) Uterine epithelium (BrdU Li) Uterine stroma (BrdU Li)... 

Experimental design The authors investigated the ability of chloroform vapors to produce toxicity and regenerative cell proliferation in female B6C3Fj mice and male Fischer 344 rats. Groups of 5 animals were exposed to 0, 1,3, 10, 30, 100, or 300 ppm chloroform via inhalation for 6 hours a day for 7 consecutive days. Actual exposure concentrations measured for mice were 0, 1.2, 3.0, 10.0, 29.5, 101, and 288 ppm and for rats were 0, 1.5, 3.1, 10.4, 29.3, 100, and 271 ppm. Necropsies were performed on day 8. Animals were administered bromodeoxyuridine (BrdU) via implanted osmotic pump for the last 3.5 days. Cell proliferation was quantitated as the percentage of cells in S-phase (labeling index = LI) measured by the immunohistochemical detection of BrdU-labeled nuclei. 

The BrdU hepatocyte labeling index values in male F344 rats given 25, 75, 150, and 300 mg/kg/day... 

For in vivo incorporation of BrdU in experimental animals, inject 10-100 mg/kg of BrdU in 0.9% saline by the ip route. A concentration of 10 mg/mL is routinely used. The animal is sacrificed after 1 h for labeling index studies or at 1-2 hourly time intervals for cell cycle progression studies In vivo incorporation of BrdU into humans at Mount Vernon Hospital involves iv injection of 200 mg of BrdU in 20 mL of normal saline as a single bolus over 3-5 min... 

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