Brain lipid-binding protein

A. Ligand Specificity of Brain Lipid-Binding Protein... 

LZ Xu, R Sanchez, A Sail, N Hemtz. Ligand specificity of brain lipid binding protein. I Biol Chem 271 24711-24719, 1996. 

After whole-body autoradiography to study the distribution of " C-labeled chloroform in mice, most of the radioactivity was found in fat immediately after exposure, while the concentration of radioactivity in the liver increased during the postanesthetic period, most likely due to covalent binding to lipid and protein in the liver (Cohen and Hood 1969). Partition coefilcients (tissue/air) for mice and rats were 21.3 and 20.8 for blood 19.1 and 21.1 for liver 11 and 11 for kidney and 242 and 203 for fat, respectively (Corley et al. 1990). Arterial levels of chloroform in mongrel dogs reached 0.35-0.40 mg/mL by the time animals were in deep anesthesia (Chenoweth et al. 1962). Chloroform concentrations in the inhaled stream were not measured, however. After 2.5 hours of deep anesthesia, there were 392 mg/kg chloroform in brain tissue, 1,305 mg/kg in adrenals, 2,820 mg/kg in omental fat, and 290 mg/kg in the liver. 

In the method described below, the processes of bilayer deformation can be studied using synthetic hposomes of any defined lipid composition. Liposomes are incubated with the protein of interest and observed in the electron microscope to reveal changes in liposome conformation accompanied with protein binding. In many of our studies where we did not know the precise hpid composition to use, we have instead used Folch brain lipids (Sigma, St. Louis, MO). 

P-Bungarotoxin-binding protein (chick brain membranes) Protein-detergent-lipid Triton X-100, 0.784 84R3... 

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