Bovine microvascular endothelial cells

Oh H, Takagi H, Suzuma K, et al. Hypoxia and vascular endothelial growth factor selectively upregulate angiopoietin-2 in bovine microvascular endothelial cells. J Biol Chem 1999 274 15732-15739. 

BPMVE, bovine pulmonary microvascular endothelial cells... 

P. D. Bowman, A. L. Betz, and G. W. Goldstein. Primary culture of microvascular endothelial cells from bovine retina Selective growth using fibronectin coated substrate and plasma derived serum. In Vitro 18 626-632 (1982). 

Figure 2.5. Setup for in vitro measurement of blood-brain barrier permeability with a co-culture of bovine brain microvascular endothelial cells (BBMEC) and an astro ioma cell line, C6. The BBMEC are grown on top of a filter insert. The C6 cells are either grown on the opposite side of the filter or on the bottom of the wells. Transport across the BBMEC monolayer is measured by adding the test substance to the upper chamber and sampling from the lower chamber. The tightness of the monolayer is also characterized by the transendothelial electrical resistance (TEER). Courtesy of T. Abbruscato.

Bovine capillary endothelial cells, human microvascular endothelial cells [tCP of alkanthiols on gold surface, protein backfill 1997, 1998 [99, 101]... 

II.1.1.1.2 Cocultures of Bovine Brain Microvascular Endothelial Cells and Rat Astrocytes 525... 

Fig. 31 Rate of surface-mediated protein C activation for TM reconstituted into a poly (acrylatePC) HBM at a protein surface coverage of 170fmolcm 2 (sample) compared to confluent endothelial cell monolayers (HDMEC, human dermal microvascular endothelial cells HUVEC, human umbilical vein endothelial cells BAEC, bovine aortic endothelial cells). Reprinted with permission from [139], Copyright 2002, American Chemical Society...

K. Suzuma, H. Takagi, A. Otani, Y. Honda (1998). Hypoxia and vascular endothelial growth factor stimulate angiogenic integrin expression in bovine retinal microvascular endothelial cells. Invest. Ophthal Vis. ScL, 39(6), 1028-1035. 

Both normoxic and hypoxic bovine pulmonary microvascular endothelial cells constitutively released xanthine oxidase (EC 1.1.3.22) activity into their culture media (Partridge et al. 1992). Incubation of hypoxic or normoxic bovine pulmonary microvascular endothelial cells with oxygenated medium (95% O2) stimulated the release of xanthine oxidase activity into the extracellular medium within 5 min. The xanthine oxidase activity could not be detected in the oxygenated medium after 60 min incubation with 95 % Oj. Oestradiol (10 iM) almost completely inhibited xanthine oxidase and dehydrogenase activities in both normoxic and hypoxic rat pulmonary microvascular endothelial cells (Kayyali et al. 1999). Dexamethasone increased the activity of xanthine oxidase, and also induced xanthine oxidase promoter activation in... 

Deli et al. (2005) presented permeability data from various in vitro BBB models by measuring transendothe-lial electrical resistance (TEER) and by calculation of permeability coefficients for paracellular or transendothelial tracers. These authors summarized the results of primary cultures of cerebral microvascular endothelial cells or immortalized cell lines from bovine, human, porcine, and rodent origin. This also described the effect of coculture with astroglia, neurons, mesenchymal cells, blood cells, and conditioned media, as well as the physiological influence of serum components, hormones, growth factors, lipids, and lipoproteins on the BBB fimction. 

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