Bourquelot Biochemical Determination of Glycosides and Oligosaccharides in Plant Materials

BOURQUELOT BIOCHEMICAL DETERMINATION OF GLYCOSIDES AND OLIGOSACCHARIDES IN PLANT MATERIALS (85) 

In carrying out the hydrolysis in alcoholic solution it was observed that the hydrolysis was incomplete, and this led to the discovery of the synthesizing action of enzymes mentioned above. The enzymic synthesis then was utilized in methods for the biochemical determination of glucose and galactose by converting them to the corresponding glycosides. 

In its present stage of development, the biochemical method probably leaves much to be desired, but additional study should make it a valuable tool for the analysis of the complex mixtures occurring in plant extracts. Methods (86) for the estimation of raflSnose in the presence of sucrose have been described which depend on selective enzymic hydrolysis. 

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