Bottle test description

Testing Procedures. Bottle Test. The most widely adopted procedure for testing emulsions is the so-called bottle test method. A detailed description of the bottle test procedure is provided in Appendix A (2). Also, Chapter 3 gives information on this topic. The purpose of the bottle test is to provide information about the effectiveness of treatment chemicals for a given emulsion. 

Graded doses ofthe test substance are incorporated into broth dispensed in McCartney boules and the bottles inoculated with the test organism and incubated. The point at which no growth occurs is taken as the bacteriostatic concentration (minimum inhibitory concentration, MIC). It is essential when performing these tests to determine the size of the inoculum as the position of the end-point varies considerably with inoculum size, which should always be defined in any description of result. 

Cleveland open cup is used to determine flash and fire points of liquids with flash point above 79°C and below 400°C, such as plasticizers. Standard gives the methods of determination using manual and automatie Cleveland open cup apparatus. About 70 ml of test liquid is heated first rapidly then slowly on approaching an expected flash point. Test flame is apphed to surface to ignite vapors. Test flame is natural or bottled gas flame (full description included in the standard). Test flame is apphed first when the temperature is 28°C below expected flash point and then in 2 C intervals. 

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