Blue Death

If the corpses had indeed appeared blue, death certainly would not have been due to carbon monoxide poisoning. A bluish appearance could, however, have been an indication of death from asphyxiation, /. e., from lack of oxygen.. 

A fatal flaw in the non-Diesel version of the CO murder story is the recurrent claim that the corpses were blue. Death from gasoline engine exhaust would only have been due to carbon monoxide and could only have caused a distinctive cherry red or pink appearance. Although Pfannenstiers post-war testimony is not nearly as wild as the Gerstein Statement, nonetheless, he and other so-called eyewitnesses also repeated the claim that the corpses were bluer 11... 

SYNS BONIDE BLUE DEATH RAT KILLER COMMON SENSE COCKROACH and RAT... 

BONIDE BLUE DEATH RAT KILLER see PHPOlO BONIDE KRAB CRABGRASS KILLER see PLC250 BONIDE TOPZOL RAT BAITS and KILUNG SYRUP see RCFOOO... 

Colourless-white crystals. Saline taste. Ingestion ataxia, dizziness, anoxia, cyanosis, convulsions, coma, brown-black urine, liver and kidney damage. Produces MetHb not responsive to methylene blue death from ingestion of more than 150 g due to hypoxic damage to tissues. Effects may be delayed 12 h post-ingestion. 

Bonide blue death rat killer 3010 Boron oxide 449... 

Morris, Robert D. The Blue Death Disease, Disaster, and the Water We Drink. New York HarperGoUins, 2007. 

Blue Death (Mosaic) Ablation with Diphtheria Toxin... 

The use of the transgenic DT-A subunit has been reported only in combination with the Blue Death method (see above Lin et al. 1995) or with a specific promoter (Bellen et al. 1992 Kalb et al. 1993). DT-A is another toxin that interferes with protein synthesis, by ADP ribosylation of the translation factor EF2 (Collier 1990). Again, the B-chain of the toxin has been removed in order to render the effects of the toxin cell-autonomous. Published stocks of flies bearing a DT-A transgene are somewhat limited in their application, the expression of the toxin being either mosaic (Lin et al. 1995) or promoter-specific (Bellen et al. 1992 Kalb et al. 1993). 

Parsons and coworkers [174] have published a route to anatoxin-o, which was isolated from strains of freshwater blue-green algae Anabaena flos aqua and is responsible for the death of livestock, waterfowl, and fish [175]. The sequence started... 

Messenger RNA molecules for both subunits of the cytochrome and the two cytosolic components are detectable in unstimulated bloodstream cells. Experiments involving incubation of neutrophil suspensions with the protein synthesis inhibitor cycloheximide indicate that constitutive expression of one or more components of the oxidase is required for the neutrophil to maintain its ability to generate reactive oxidants. For example, when neutrophils are incubated in vitro with cycloheximide, their ability to generate reactive oxidants declines more rapidly than in control cells, as they age in culture (Fig. 7.12). This decline in oxidase activity when protein biosynthesis is blocked is not due to cell death, because cells treated with cycloheximide for this time still exclude trypan blue. Furthermore, when protein biosynthesis is stimulated in neutrophils by the addition of GM-CSF for 24 h in vitro, the ability to generate reactive oxidants is enhanced considerably above the levels observed in untreated cells. 

Before further testing and to confirm that the compounds are cytotoxic rather than merely interfering with the Alamar blue indicator dye, they are re-bioassayed using two other indicator dyes. Calcein-AM is a fluorescent dye that measures changes in cell mem brane permeability, an indicator for one of the penultimate steps of cell death, uci e e measures the amount of adenosine triphosphate (ATP) synthesis in a chemilurmne assay. For some compounds, cell death was also confirmed by microscopic exami Papanicolaou-stained cell preparations.11... 

Figure 1. Inhibition of geranylgeranoic acid-induced apoptotic cell death by a-tocophrol. Increasing concentrations (10 100 pM) of a-tocopherol were added to HuH-7 cell cultures with (closed circle) or without (open circle) 10 pM geranylgeranoic acid. After overnight incubation, the number of the viable cells was counted using a Trypan blue dye exclusion method. Means S.E. (n=3) are shown.

These nebulas are similar in some respects to the Hll regions. The difference is that here the source of ionisation is an ageing star (white dwarf) in its death throes rather than a strapping young blue star. The fluorescent region is both denser and chemically more complex for it includes those atoms expelled from the envelope of the dying star in the form of a stellar wind. 

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