Bladder smooth muscle cells

Figure 16.14 Original Kca1-1 (BK) single channel recordings and the inhibitory effect of iberiotoxin. Currents were recorded from a guinea pig urinary bladder smooth muscle cell using the whole cell (cell-attached), perforated patch-clamp technique at 0 mV holding potential (Section...

Figure 16.16 Data from Kca1-ipi-subunit knockout (KO) mouse urinary bladder smooth muscle cells Indicate that the Kca1-1 (BK) channels from pi-subunlt KO mice have a reduced open probability compared to control (normal wild type) mouse. (A) Illustrates single channel recordings from excised Inside-out membrane patches (Section 16.5.1.3.) held at 40 and -i-40 mV In 10 gM free Ca + and symmetrical 60 gM K+ concentration. Arrows indicate the closed state of the channels. (B) illustrates BK channel open probability in control and pi-subunit KO animals at two different voltages (-40 and +40 mV). Data were obtained from n=9 11 cells of each animal group. Asterisks indicate statistically significant difference. Used with permission from Petkov et al., 2001a, J. Physiol. 537 443-452 - Copyright 2001 The Physiological Society.

Elevated NGF concentrations have been seen in two conditions involving altered muscle morphology. Hypertrophy and hyperplasia of bladder smooth muscle cells induced by increased in-travesicular pressure elevates NGF content 2-fold (Steers et al., 1991), while, within the mesenteric artery of the SHR, a rise in NGF concentration correlates with the onset of smooth muscle hyperplasia (Zettler and Rush, 1993). Since abnormalities also occur within the innervation of both these pathologies, it is unclear whether the altered NGF concentrations result from changes in muscle or nerve function. 

Thapa, A. et al.. Nano-structured polymers enhance bladder smooth muscle cell function. Biomaterials, 2003,24 2915-26. 

Thapa, A., T.J. Webster, and K.M. Haberstroh, Polymers with nano-dimensional surface features enhance bladder smooth muscle cell adhesion. / Biomed. Mater. Res., 2003,67A 1374—83. 

Bisson I, Kosinski M, Ruault S, Gupta B, Hilbom J, Wurm F, et al. Acrylic acid grafting and collagen immobihzation on poly(ethylene terephthalate) surfaces for adherence and growth of human bladder smooth muscle cells. Biomaterials 2002 23 3149-58. 

R Xu, Y. Wang, X. Jiang, H. Tan, H. Li, K.J. Wang, Effects of different biomaterials comparing the bladder smooth muscle cells on waterborne polyurethane or poly-lactic-co-glycolic acid membranes, Kaohsiung J. Med. Sci. 28 (January 2012) 10-15. 

M.A. Pattison, T.J. Webster, K.M. Haberstroh, Select bladder smooth muscle cell functions were enhanced on three-dimensional, nano-structured poly(ether urethane) scaffolds, J. Biomater. Sci. Polym. Ed. 17 (2006) 1317-1332. 

Danielsson C, Ruault S, Basset-Dardare A, Frey P. Modified collagen fleece, a scaffold for transplantation of human bladder smooth muscle cells. Biomaterials 2006 27 1054-60. 

Peptide nanofibers Bladder tissue Promotes bladder smooth muscle cells attachment. Harrington et al. 

Park, J.M., T. Yang, L.J. Arend, J.B. Schnermann, C.A. Peters, M.R. Freeman, and J.P. Briggs. 1999. Obstruction stimulates cox-2 expression in bladder smooth muscle cells via increased mechanical stretch. American Journal of Physiology 276(1 Pt 2) F129-36. 

AzzareUo, J., B. R Kropp, K. M. Fung, and H. K. Lin. 2009. Age-dependent vascular endothelial growth factor expression and angiogenic capabiUty of bladder smooth muscle cells Implications for cell-seeded technology in bladder tissue engineering. / Tissue Eng Regen Med 3 579-589. 

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