Biosynthetic combinatorial libraries peptide

Combinatorial approaches in synthetic chemistry allow the high-throughput screening of millions of compounds. It is the aim to establish such approaches in biosynthetic systems by creating module assemblies, which provide a new tool to create peptide libraries. The large number of different modules in peptide synthetase systems is suitable for creating a pool of many different enzymes in order to detect a useful compound in the near future [2,51],... 

Because these genes are often arranged in clusters, this has allowed a combinatorial biosynthetic approach for the construction of diverse libraries of polyketides and nonribosomal peptides.388387 The use of E. coli as a production host whereby precursor supply and selected pathway enzymes are modified has resulted in respectable titers of targeted compounds as well as novel derivatives.388393... 

Recently, combinatorial technologies and libraries have somewhat replaced NPs as a source of diversity in pharmaceutical research. A comparison of combinatorial technologies with the fermentation of an NP-producing organism show that they both produce a library of compounds. While the former library is structurally determined a priori either by the selected synthetic scheme or by recombinant genetic information in the case of biosynthetic peptide and ON libraries, the latter is the result of the metabolic complexity of the producing strain and has to be deconvoluted in order to... 

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