Biomolecules, glycosylated

Glycosyl Phosphates Deoxy sugars, which are vital components of various biomolecules such as vancomycin, erythromycin and daunomycin are difficult to be... 

The protein molecular mass is insufficient information for identification, but it is adequate to confirm identity therefore, MS is one of the preferred techniques for characterization and quality control of recombinant proteins and other biomolecules. In the same way, it has been used to study posttranslational modifications (like glycosylation and disulfide bonding pattern), and other processes that can modify protein mass.11... 

As is quite apparent, the topics that appear within this volume vary quite extensively. However, whether it is a discussion of the transfer of an oligosaccharyl moiety to a serine residue in 0-glycosylation or the regulation of transcription termination through a riboswitch, it is hoped that the reader will not only come away with an appreciation for all of the biomolecules discussed, but more importantly recognize the diversity of processes that these small molecules and associated polymers can perform and/or regulate. 

The delicate nature of biomolecules such as (glycosylated) proteins, polysaccharides and metabolites necessitates appropriate methods for purification and separation, such as chromatography, lyophilization and crystallization. 

After the initial fervor about microbial expression of recombinant DNA (rDNA) proteins, it was realized that this method was not suitable for large proteins that needed to be correctly folded to ensure desired activity. Animal cells, on the other hand, have this ability to fold and process such extremely intricate proteins (Lubiniecki 1998). For instance, proteins that require substantial glycosylation as well as appropriate folding or need a proper folding environment owing to the abundance of disulfide bonds are best derived from animal cells. Bacterial cellular machinery does not have the ability to make complex biomolecules such as proteins, which require... 

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