Bioluminescence imaging detection

Chen, X.J., Zhang, X.M., Larson, C.S., Baker, M.S., Kaufman, D.B. In vivo bioluminescence imaging of transplanted islets and early detection of graft rejection. Transplantation 81, 1421-1427 (2006)... 

Fig. 3 HPTLC bioluminescence image of the same plate as in Fig. 2. Note that the different numbers of zones in the chromatograms detected in some of the lanes by the two methods provide complementary information on the samples. Source Photograph supplied by Camag, Muttenz, Switzerland.

Figure 10.3 (See color insert) Bioluminescent imaging of a wild-type mouse after implantation of a light emitting bead (with a spectrum similar to that of firefly luciferase) in the pleural space posterior to the right lung (a), in the pleural space anterior to the right lung (h), or anterior to the rih cage (c). The pseudocolor scale is shown and the region of interest (ROI) for photon detection is indicated by a red circle. Detected photon counts are indicated for each ROI.

Figure 2 Imaging protein-protein interactions by FRET and BRET, (a) Diagram illustrating FRET between the donor CFP-fusion and the acceptor YFP-fusion. (b) Detection of protein-protein interaction between X and Y by BRET using the Renilla luciferase (Rluc) as a bioluminescent donor and GFP as a fluorescent acceptor. The substrate for Rluc is deep blue coelenterazine. Constructs not drawn to scale.

Tbe luminescence of GGA solutions were estimated using the bacterial chip system in order to produce a calibration plot for measurement of pollution containing biodegradable substances. The luminescent intensity was correlated with concentration of BOD standard (GGA) solution. The bioluminescence increased linearly with concentration up to approximately 50 ppm. Measurement of BOD values less than 50 ppm has been achieved. The bght intensity reached saturation at concentrations of over 100 ppm. Detection limit was approximately 1(X) ppm, and the minimum measurable BOD was 1 ppm. Based on the logarithmic curve which was obtained by the measurement with a chemi-imager, two linear calibration curves and equations were estimated and approximated... 

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