Biochemical Syntheses in the Sucrose Series

It had been known from at least the time of Pasteur that the presence of sodium or potassium phosphate aided the progress of a yeast fermentation. Later intensive study showed that a complex group of enzymes (phosphatases and phosphorylases) was responsible for the phosphorylation, dephosphorylation and interconversion of D-glucose 6-phosphate, D-fructose 6-phosphate, D-fructose 1,6-diphosphate and similar substances in various types of cells and muscle tissue. Detailed reviews of the field are available. - A further advance was made in 1936, when Cori and Cori noted that in certain circumstances well-washed frog muscle immersed in a sodium phosphate buffer utilized the inorganic phosphate to produce a new hexose phosphate (the Cori ester). This compound was later shown to be a-D-glucopyranose-l-phosphate and yielded crystalline dipotassium and brucine salts. The Cori ester arose because 

Cori ester + D-fructose sucrose + inorganic phosphate, 

The suggestion was that the components on either side of the equilibrium united, without the intervention of water, to form the sucrose-phosphoric acid adduct XX, which decomposed in either direction as shown by the dotted lines. 

Bray and M. Stacey Departments of Chemistry and Physiology, The University, Birmingham, England 

Blood Group Substances from Other Sources. 42 

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