Bienzymatic process

Enantioconvergent biohydrolytic transformation of paraoxide using a bienzymatic process. The sequential use of Solanum tuberosum and Aspergillus niger EHs as biocatalysts led to the formation of enantiopure (/ )-para-chlorophenyl-1,2-diol, a chiral building block for the synthesis of (/ )-eliprodil. 

Mass spectrometry has been implemented as an on-line detection tool to monitor the transmission of chemical signals due to natural processes such as diffusion and convection as well as a bienzymatic autocatalytic process [6]. Using a mass spectrometer as the detector, it was found that an enzyme-accelerated chemical wave propagates faster than a chemical wave propelled by other processes. The two enzymes involved in the process (pyruvate... 

Finally, Griengl s group has developed the synthesis of aromatic 1,2-amino alcohols on the basis of a bienzymatic DYKAT process. The reaction occurred between a benzaldehyde derivative and glycine in the presence of L-threonine aldolase from Pseudomonas putida and L-tyrosine decarboxylase from either Enterococcus faecalis or two genes from Enterococcus faecium. The best results were obtained for the production of (5)-octopamine (99%, ee = 81%), and (5)-noradrenaline (76%, ee = 79%), as shown in Scheme 3.64. 

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