Bienzymatic Process Implying One Epoxide Hydrolase

Styrene monoxygenase from E. coli JMlOl pSPZlO 5 g/l resting cells 

TABLE 8.5 Enantioselective 5- and / -Specific Dihydroxylation of Styrene Oxide and Some of Its Monosubstituted Derivatives 

Cells Anal. Yield % Diol Abs. Conf./ ee % Anal. Yield % Diol Abs. Conf./ee % Anal. Yield % Diol Abs. Conf./ee % Anal. Yield % Diol Abs. Conf./ee % 

In order to demonstrate the usefulness of the developed process as a synthetic chemistry tool, the substrate and the biomass concentrations were increased to 50mM and 20g cdw/1, respectively the performance of the biotransformation set up was tested with some of the best substrates using either the SSPl or SSTl cells. Very good isolated yields as well as high selectivities were determined, enabling the 

8 EPOXIDE HYDROLASES AND THEIR APPLICATION IN ORGANIC SYNTHESIS 

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