Bessey-lowry activity

Bessey-Lowry Activity One unit of activity may be defined as the amount of enzyme present in 1 millilitre of serum that liberates lp mol of / -nitrophenol (0.1391 mg) per horn at pH 10.5 after 30 minutes at 38°C. 

Since a number of methods are in widespread use, it is worthwhile to review comparative studies of alkaline phosphatase activity measured by these different methods. Schwartz et al. (S24) established that a linear relation exists between the results of the Bodansky and King-Armstrong methods. Laurent and Norberg (L5) also demonstrated a linear relation between values on serum performed with a modified Bessey-Lowry compared to a modified King-Armstrong method. However, Gelb et al. 

In some methods, substrates are used which yield coloured products, e.g. p-nitrophenylphosphate (in the Bessey-Lowry-Brock method), thymolphthalein phosphate or phenolphthalein phosphate. These substrates can be used for the kinetic measurement of enzymic activity, unlike the previous methods which are end-point assays. 

Alkaline phosphatase (ALP) activity ALP activity of the supematarrt was meastrred using an ALP B-Test WAKO kit (Wako, Japan) based on the Bessey-Lowry method. ALP activity was normalized by samples total protein production measured with a Bicinchoninic Acid Assay (BCA, BCA Protein Assay KiL Pierce Biotechnology, U.S. A.). 

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See also in sourсe #XX -- [ ]

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