Batch rebinding experiments

The data are average results of three independent batch binding experiments where the association constants K, have been determined from the linear regions of the corresponding Scatchard plots. The site classes have been identified from a biphasic behaviour of the plots. P(EA) refers to a MIP imprinted with 9EA and P(BA) to a MIP imprinted with BA. Data from [24]. 

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Adsorption-desorption kinetics in batch rebinding experiments... 

L-aspartic acid L-aspartic acid-imprinted chitosan evaluation by batch rebinding experiments Monier et al., 2010... 

This type of interaction has now been used to prepare a variety of imprinted polymers where A-toluoyl-glycyl-L-valine methyl ester acted as chiral template and 3-trifluoroacetylamino-5-(4-styryl)pyrazole 23f as the binding site monomer. In batch rebinding experiments, some of them showed a pronounced enantioselectivity towards rebinding of template (up to a = 2.7). The re-uptake of substrate to the polymers was generally only low. 

Table 3 Small-scale Batch Rebinding Experiments for MiniMIPs Prepared using the Drug Lead E (Pe) and Its Structural Analogue F (Pp) as Template...

Binding isotherms are commonly measured in MlPs from batch rebinding [4,5] or frontal chromatography studies [6-8]. In batch rebinding experiments, a constant weight of polymer is equilibrated with solutions of analyte of varying initial concentration (I) (Fig. 2). Equilibration of the solution typically takes anywhere from 15 min to 24 h and should be independently measured for each new polymer. The... 

For determining the adsorption isotherm, the equilibrium concentrations of bound and free template must be reliably measured within a large concentration interval. Since the binding sites are part of a solid, this experiment is relatively simple and can be carried out in a batch equilibrium rebinding experiment or by frontal analysis. 

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