Growth batch

Fig. 11.3. Batch growth curve with various phases.

Under ideal batch growth conditions, the quantity of biomass, and therefore the biomass concentration will increase exponentially with respect to time and in accordance with all cells having the same probability to multiply. Thus the overall rate of biomass formation is proportional to the biomass itself where... 

Batch growth was started by inoculating the fermenter with 100 mL of a freshly grown preculture of E. coli JMlOl (pSPZlO) (see Procedure 2, Section 12.8.2). The pH was kept at 7.1, the aeration rate was set to 2 L min and the stirrer speed and temperature were 1300 rpm and 30 °C respectively. 

An increase in the spread of sizes in a batch crystallization could be due to size dependent growth or also growth dispersion. Whatever the cause, the spreading decreases substantially for the high alcohol content crystallizations. There seems to be no current explanation for this effect. In practical terms, this means that batch growth from alcoholic solutions should give a more uniform product than from aqueous solution. 

Figure 3. Changes in size distribution with time during growth of ice crystal seeds in 6% lactose under batch or semi-batch growth conditions, concentration processes based on suspension growth. In addition, the relation of this observed widening to the heat balance conditions needs to be further explored.

Fig. 5.77. Batch growth curve produced by the model of Ramkrishna et al.

According to Eq. (6.21), the batch growth time t - tQ is the area under the l/rx versus Cx curve between Cx and Cx as shown in Figure 6.5. The solid line in Figure 6.5 was calculated with the Monod equation and the shaded area is equal to t -10. The batch growth time is rarely estimated by this graphical method since the Cx versus t... 

Kinetic and Stoichiometric Parameters of D. hansenii CCMI 941 Batch Growth in Nonsupplemented and Supplemented Nondetoxified, Activated Charcoal Detoxified and Anion-Exchange Resins Detoxified BSG Dilute-Acid Hydrolysates ... 

Fig. 1. Batch growth and antibody production of Nicotiana tabacum (BY-1) cells in terms of pg 1 1 protein, wet cell concentrations, and dry cell concentrations. Dashed vertical lines represent the boundaries between lag, exponential, and stationary growth phases. The 4-day offset between peak concentrations for biomass and protein product indicates that growth and product formation are not directly linked...

Fig. 10. PCA plot of a perfusion culture with CHO cells producing recombinant hFVIII. The data points in the plot mirror inoculum of the bioreactor and a phase of batch growth (c), transfer from growth to production medium (D), production phase of rFVIII (demarcated with dotted lines) and the deviation of a bacterial infection of the cell culture (E) (from Bachinger et al.[37], with permission)...

Erickson et al. (E3) developed a model for batch growth in fermentations with two liquid phases present in which the growth-limiting substrate is dissolved in the dispersed phase. The model accounts for drop size distribution and considers the effect of droplet coalescence and redispersion by an interaction model similar to that of Eq. (110). Droplet interactions were shown to be important if drop size distributions have large variance. 

The best way to characterize cell metabolic patterns is in terms of consumption rates and production rates per viable cell (collectively termed metabolic quotients). This allows much easier comparison between different cell types or culture conditions than do plots of changes in the levels of nutrients or products over time. It also shows directly how metabolic patterns change with time during batch growth... 

The general formula for for batch growth in a stirred vessel is given by Equation 4.2.32, where P is the extracellular product concentration. Frequently, q is relatively constant during batch growth. One example is monoclonal antibody production by hybridoma cells (Renard et al, 1988). If it is assumed that q does not vary with time, then Equation 4.2.32 can be integrated to yield Equation 4.2.45 ... 

Figure 1.22. Biomass concentration during batch growth.

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