Basic compounds liquid chromatographic separations

The two basic questions in high-performance liquid chromatography focus on (a) how particular compounds can be separated, and (b) why particular compounds were separated by the liquid chromatographic method used. The answers can be obtained by the consideration of some simple representative chromatograms of the separation of well-known compounds. Such separations can be easily understood according to common principles of physics and chemistry. 

Amadori compounds (N-substituted-l-amino-l-deoxy-2-ketoses) are potential precursors to the formation of many of these heterocyclic volatile products. The secondary nitrogen in most Amadori compounds is weakly basic and is therefore a likely site for rapid nitrosation reactions via normal reactions with nitrous acid, under mildly acidic conditions. However, purified Amadori compounds are usually obtained only after tedious isolation procedures are invoked to separate them from the complex mixtures of typical Maillard browning systems. Takeoka et al. ( 5) reported high performance liquid chromatographic (HPLC) procedures to separate Amadori compounds in highly purified form on a wide variety of columns, both of hydrophilic and hydrophobic nature. They were able to thus demonstrate that reaction products could be followed for kinetic measurements as well as to ensure purity of isolated products. 

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