Bacteria selective media

Once the bacteria have grown on the non-selective medium, cultures should be examined for their colonial morphology, and also to determine whether the organism has had any effect on the medium. Generally cultural characteristics on simple media are too variable to be of diagnostic value and, although great... 

Lee s multidifferential medium (LMD) t General non-selective medium. Acid-producing bacteria detected by dissolution of calcium carbonate present in medium and by colour change of bromocresol green indicator. 

Figure 3 Root fingerprints of Pseudomimets sp. associated with barley seedlings showing the production of siderophore by actively growing bacteria located in the zone of elongation behind the root tips. Root.s were pressed on to an iron-deficient minimal medium selective for Pseudomonas. After growth of the colonies, the production of siderophore was visualized by exposure of the agar plate to ultraviolet light, which causes the siderophore to Huoresce.

In the approach followed in this invention [29], a biocatalytic agent converts the sulfur heterocycles into different molecules that do not exhibit the hydrophobic interactions. This is achieved by selectively cleaving carbon-sulfur bonds. The selectivity of the biocatalytic agent employed is limited to the carbon-sulfur bonds and no attack to the carbon-carbon skeleton was reported. Thus, it is expected that the proposed biocatalytic reduction of viscosity would not diminish the fuel value of the treated petroleum liquids. The biocatalyst employed consisted of the strain ATCC No. 53968 (see Section 20 and references therein), in an aqueous culture conventionally prepared by fermentation under aerobic conditions. The fermenting bioreactor is fed with a suitable nutrient medium, which comprises a conventional carbon source (dextrose and glycerol are recommended carbon sources. To confer maximal biocatalytic activity for the desired cleavage of organic C—S bonds, the bacteria was kept in a state of sulfur deprivation. 

For the production of PHB, a specially selected bacteria is used. They grow in aqueous media during air injection at 35°C. At the end of the fermentation process about 80% of cell dry weight consists of PHB. Production is about 100 kg PHB/m of medium (Fig. 7). 

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