Bacillus atrophaeus

Czerwieniec, G. A. Russell, S. C. Tobias, H. J. Fergenson, D. P. Steele, P Pitesky, M. E. Horn, J. M. Frank, M. Gard, E. E. Lebrilla, C. B. Stable isotope labeling of entire Bacillus atrophaeus spores and vegetative cells using bio-aerosol mass spectrometry. Anal. Chem. 2005, 77,1081-1087. 

Fig. 17. Germination curves of typical aerobic (Bacillus atrophaeus) and anaerobic (Clostridium sporogenes) spores. (Data provided by W.-W. Yang.)...

Demirev et al. reported the identification of proteins and their source microorganism Bacillus atrophaeus and Bacillus cereus) using a MALDI-TOF-TOF-MS/ MS and top-down proteomic identification with software developed in-house. The software compares the m/z of MS/MS fragment ions to the m/z of in silico fragment ions (a, b, y, and loss of NH3 or H2O) derived from bacterial protein sequences having the same MW as the protein biomarker. A P-value algorithm was also developed to score/rank identifications based on a number of parameters including the number of MS/MS in silico matches [17],... 

A laser ablation TOF mass spectrometer has been developed to iderrtily individual airborne micrometer-sized particles, comprising a single cell or a small mrmber of clumped cells (Tobias et al. 2005). This approach is reagerrt-less, and it relies on laser ablation and detection of lower mass (less than m/z 200) positive and negative ions. MS signatrrres for aerosolized Mycobacterium tuberculosis particles are distinct fromM smegmatis. Bacillus atrophaeus, and B. cereus particles. This technique is tested as a stand-alone airborne M. tuberculosis detector in bioaerosols from an infected patient at airborne concentrations of 1 particle/liter. 

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