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BAC clones

Studies of overall genome composition based on reassociation kinetics (Simpson et ai, 1982 Cox et ai, 1990 Marx et a/., 2000) and analysis of fully sequenced bacterial artificial chromosome (BAC) clones from the 5. mansoni genome project show that platyhelminth genomes contain abundant highly and moderately repetitive sequence (Fig. 2.1). Much of the repetitive DNA comprises two classes of integrated mobile elements class I elements, which include long terminal repeat (LTR) retrotransposons and retroviruses, non-LTR retro-transposons and short interspersed nuclear elements (SINES) and transpose via an RNA intermediate, and class II elements (trans-posons), which transpose as DNA (Brindley et ai, 2003). Additionally, small dispersed or tandemly repeated sequences are common. A wide variety of these sequences have been isolated and characterized from a variety of taxa (Table 2.4). [Pg.43]

Mus musculus BAC clone RP23-344I7 from chromosome 18 26/27 0.015... [Pg.549]

Mus musculus BAC clone RP24-267I18 from chromosome 5 21/21 0.23... [Pg.549]

The large DNA band containing the insert of the PI or BAC clone is excised from the nnstained portion of the gel and equilibrated for 30 min at room temperatnre in 25 ml Gelase bnffer (Epicentre Technologies, Madison, Wl) snpplemented with 100 mM NaCl, 30 pM spermine, and 70 pM spermidine. [Pg.171]

Figure 10.2. XGRAIL output using the human BAC clone RG364P16 from 7q31 as the query. The upper window shows the results of the prediction, with the histogram representing the probability that a given stretch of DNA is an exon. The various bars in the center represent features of the DNA (e.g., arrows represent repetitive DNA, and vertical bars represent repeat sequences). Exon and gene models, protein translations, and the results of a genQuest search using the protein translation are shown. (See color plate.)... Figure 10.2. XGRAIL output using the human BAC clone RG364P16 from 7q31 as the query. The upper window shows the results of the prediction, with the histogram representing the probability that a given stretch of DNA is an exon. The various bars in the center represent features of the DNA (e.g., arrows represent repetitive DNA, and vertical bars represent repeat sequences). Exon and gene models, protein translations, and the results of a genQuest search using the protein translation are shown. (See color plate.)...
Figure 10.6. GENSCAN output in graphical form, using the human BAC clone RG364P16 from 7q31 as the query. Optimal and suboptimal exons are indicated, and the initial and terminal exons show the direction in which the prediction is being made (5 3 or 3 5 ). Figure 10.6. GENSCAN output in graphical form, using the human BAC clone RG364P16 from 7q31 as the query. Optimal and suboptimal exons are indicated, and the initial and terminal exons show the direction in which the prediction is being made (5 3 or 3 5 ).
Pick an isolated colony of DHIOB cells containing the desired BAC clone and grow overnight in 3 ml of Superbroth special media at 32°C. [Pg.116]

See other pages where BAC clones is mentioned: [Pg.250]    [Pg.184]    [Pg.8]    [Pg.318]    [Pg.318]    [Pg.66]    [Pg.67]    [Pg.236]    [Pg.148]    [Pg.67]    [Pg.398]    [Pg.120]    [Pg.120]    [Pg.41]    [Pg.146]    [Pg.86]    [Pg.86]    [Pg.267]    [Pg.175]    [Pg.470]    [Pg.198]    [Pg.199]    [Pg.243]    [Pg.258]    [Pg.699]    [Pg.980]    [Pg.129]    [Pg.238]    [Pg.238]    [Pg.240]    [Pg.124]    [Pg.82]    [Pg.11]    [Pg.12]    [Pg.21]    [Pg.155]    [Pg.177]    [Pg.180]    [Pg.44]    [Pg.114]    [Pg.82]   
See also in sourсe #XX -- [ Pg.86 ]

See also in sourсe #XX -- [ Pg.86 ]



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Bacterial artificial chromosome BAC) clones

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