B Group Vitamins in Honey

Due to the low concentration of B group vitamins in honey, the measurement of the analyte requires a very sensitive method. Colorimetric, spectrophotometric, spectrofluorimetric, microbiological and reversed-phase HPLC (RP-HPLC) analytical approaches have been used until now. 

Microbiological methods used for the determination of B group vitamins in honey. 

While data published in the literature are quite homogeneous regarding the amount of B group vitamins in honey, the reported concentration of vitamin C in honey varies widely over several orders of magnitude. 

Key Facts about Assaying B Group Vitamins in Honey... 

The data reported in the literature almost exclusively concern water-soluble vitamins (B group vitamins and vitamin C). For this reason, this chapter limits its treatment of the analytical aspects of honey to these compounds. 

Unlike what is observed with B group vitamins, the concentration of vitamin C in honey is spread over many orders of magnitude. 

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