Azo dyes Reactive Red

Beydilli MI, Pavlostathis SG, Tincher WC (2000) Biological decolorization of the azo dye Reactive Red 2 under various oxidation-reduction conditions. Wat Env Res 72 698-705... 

Maas R, Chaudhari S (2005) Adsorption and biological decolourization of azo dye Reactive Red 2 in semicontinuous anaerobic reactors. Process Biochem 40 699-705... 

Metabolites formed during the decolourization of the azo dye Reactive red 22 by Pseudomonas luteola were separated and identified by HPLC-DAD and HPLC-MS. The chemical structures of Reactive red 22 (3-amino-4-methoxyphcnyl-/fhydroxyl-sulphonc sulphonic acid ester) and its decomposition products are shown in Fig. 3.92. RP-HPLC measurements were carried out in an ODS column using an isocratic elution of 50 per cent methanol, 0.4 per cent Na2HP04 and 49.6 per cent water. The flow rate was 0.5 ml/min, and intermediates were detected at 254 nm. The analytes of interest were collected and submitted to MS. RP-HPLC profiles of metabolites after various incubation periods are shown in Fig. 3.93. It was concluded from the chromatographic data that the decomposition process involves the breakdown of the azo bond resulting in two aromatic amines [154],... 

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