Auxin Duboisia hybrid root culture

The effect of auxins on the growth and the alkaloid production in Duboisia hybrid root cultures... 

The adventitious root cultures of Datura innoxia, Duboisia hybrid M-II-8-14 (a cross-bred between D. myoporoides and D. leichhardtii), and Scopolia tangutica were established from the axenic shoot cultures or intact plants (in the case of Duboisia) on MS solid medium containing 0.1 mg/1 NAA or 1.0 mg/1 lAA. The adventitious roots were maintained in MS liquid medium containing the same phytohormones (0.1 mg/1 NAA or 0.5 mg/1 lAA) in the dark. Addition of auxin in the culture medium has been employed for the maintenance of adventitious root cultures [14], however, the adventitious roots of H. albus and H. niger were induced and maintained in hormone-free 1/2 MS medium [15]. 

Callus and root organ cultures of Duboisia have been studied with a view to develop a new method to obtain tropane alkaloids more efficiently [64-66]. In solanaceous plants, which produce tropane alkaloids, cultured roots produce them but calli do not [65] or only in very small amounts [66]. Root organ culture might be a useful method to produce tropane alkaloids and suitable culture conditions for growth and alkaloid production must be established. In this section, the effects of auxins including lAA chloro-derivatives on the growth and alkaloid yields in root organ cultures of Duboisia myoporoides - D. leichhardtii hybrid are described [67, 68]. 

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