Autoradiography resolution

Compounds that are radioactive can be located on a preparative layer by contact film autoradiography, electronic autoradiography, and storage phosphor screen imaging [21-23]. These methods differ in terms of factors such as simplicity, speed, sensitivity, and resolution, and the method of choice depends on the available equipment, reagents, and instrumentation. All are nondestructive, and the detected compounds can be recovered without change for later studies. 

Vanha-Pertulla T, Grimley PM. Loss of proteins and other macromolecules during preparation of cell cultures for high resolution autoradiography quantitation. Histochem Cytochem 1970 18 565-573. 

Administration of Radioisotopes, Tissue Preparation, and Emulsions Resolution and Quantitative Autoradiography Limitations of EM Autoradiographs Protocols Conclusions References... 

Bouteille M, Dupuy-Coin AM, Moyne G. Techniques of localization of proteins and nucleoproteins in the cell nucleus by high resolution autoradiography and cytochemistry, in Methods in Enzymology (O Malley BW, Hardman JG, eds.), Academic Press, New York, 1975, pp. 3-41. 

Stumpf WE, Roth LJ. 1966. High resolution autoradiography with dry mounted, freeze-dried frozen sections. Comparative study of six methods using two diffusible compounds H-estradiol and H-mesobilirubinogen. J Histochem Cytochem 14 274-287. 

Fakan, S. and Bernhard, W. (1971) Localisation of rapidly and slowly labelled nuclear RNA as visualized by high resolution autoradiography. Exp. Cell. Res. 67(1), 129-141. 

M.T. Wyss, B. Weber, M. Honer, N. Spath, S.M. Ametamey, G. Westera, B. Bode, A.H. Kaim, A. Buck, F-choline in experimental soft tissue infection assessed with autoradiography and high-resolution PET, Eur. J. Nucl. Med. Mol. Imaging 31(3) (2004) 312-316. 

Distribution studies with radiolabeled test substances in animals are an important part of the drag development process. Traditional routine methods used for these studies are quantitative tissue distribution studies (QTD) and alternatively whole-body autoradiography (WBA) with detection of the radioactivity in whole-body sections on X-ray film (John R. J. Baker 1989). WBA is a qualitative detection method with a very high local resolution which includes all organs an many small substructures. 

Radiolabeled ligands have also enabled development of the technique of autoradiography, a method of great sensitivity that can provide anatomical resolution in the micron range and hence yield evidence about the location of receptors superior to that derived from binding experiments.0 ... 

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