Association constants concanavalin

Ac, acetyl AONs, antisense oligonucleotides B, boat Bn, benzyl Bz, benzoyl C, chair CD, circular dichroism CO, carbon monoxide ConA, concanavalin A DAST, diethylaminosulfur trifluoride DFT, density functional theory DMDO, dimethyldiox-irane DMT, dimethoxytriphenylmethyl DNA, deoxyribonucleic acid dsDNA, double-stranded DNA E, envelope Fmoc, fluorenylmethyloxycarbonyl GlcNAc, /V-acetylglucosamine ITC, isothermal titration calorimetry kcat, catalytic rate constant Aa, association constant K, inhibition constant KM, Michaelis constant LiSPh, lithium thiophenolate LPS, lipopolysaccharide pM, micromolar MMT,... 

Tab. 1 Association constants of 5 to the photoisomerizable Concanavalin A systems (3)-Con.A and (4)-Con.A. as a function of the degree of loading.

The separation of lectins from lima beans (Phaseolus lunatus) on a column of concanavalin A-Sepharose has been reported binding studies with methyl 2-acetamido-2-deoxy-a-D-galactopyranoside showed that the association constants of the lectins are virtually identical. ... 

Willner et al. attached a thienylfulgimide to concanavalin A, a protein that forms complexes with pyranoses such as a-D-mannopyranoside, to form 31. UV irradiation increased the association constant. Also, a thienylfulgide-modified a-chimotrypsin 32 was synthesized by Willner et al. The esterification rate was controllable by photoirradiation. 

To mimic saccharide-protein interactions, some PEG-polyrotaxanes were synthesized in Yui s group [38-40]. In these polyrotaxane, many binding ligands were introduced to the CD rings. High association constants were found between these polyrotaxanes and proteins such as concanavalin A and streptavidin. 

The self-association of solutions of concanavalin A at pH 7 has been shown to be temperature dependent the lectin is essentially subject to a reversible dimer-tetramer transition below 23 C. From the measurement of various kinetic parameters of the transition and calculation of thermodynamic constants, it is concluded that the two dimers associate through a large hydro-phobic binding region. 

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