Assays purification

Stults, N. L., et al. (1992). Use of recombinant biotinylated aequorin in microtiter and membrane-based assays Purification of recombinant aequorin from Escherichia coli. Biochemistry 31 1433-1442. 

Volume 200. Protein Phosphorylation (Part A Protein Kinases Assays, Purification, Antibodies, Functional Analysis, Cloning, and Expression)... 

Hastings J.W., Baldwin T.O., Nicoli M.Z., Bacterial luciferase assay, purification and properties, Methods Enzymol. 1978 57 135-152. 

Specific Assays, Purification, and Study of Structure—Activity Relationships of Cellulolytic... 

The present article describes the occurrence, assay, purification, and properties of L-arabinosidases, classified into exo- and endo-types. Unless otherwise noted, the arabinosides discussed are in the L-furanoid form. 

Barker, H. A., Smyth, R. D., Weissbach, H., Munch-Peterson, A., Toohey, J. I., Ladd, J. N., Volcani, B. E. and Marilyn Wilson, R. 1960A. Assay, purification, and properties of adenylcobamide coenzyme. J. Biol. Chem. 235, 181-190. 

Pectate Hydrolases (Miscellaneous) and Lyases The assay, purification, occurrence, action patterns, and specificities of pectate hydrolases and lyases have been reviewed. ... 

Nucieotidases.—Since the 5 -nucleotidase from Dictyostelium discoideum interacted with immobilized concanavalin A, it appears to be a glycoprotein. Pectinesterases.—The occurrence, formation, assay, purification, and specificities of pectinesterases have been reviewed. Pectinesterase activity was detected both in healthy and diseased onions Allium cepa), but not in cultures of the invading bacterium (Pseudomonas cepacia) Both the pectinesterase and poly-D-galact-uronate lyase activities of Clostridium multifermentans are associated with a single complex. ... 

Independent Assays for Provings Virus Removal. Retrovimses and vimses can also be present in culture fluids of mammalian cell lines (15,24). Certainly the absence of vims can be difficult to prove. Model vimses, eg, NIH Rausher leukemia vims and NZB Xenotropic vims, were spiked into fluids being purified, and their removal subsequently vaUdated when subjected to the same purification sequence as used for the product. 

Biomedical Applications. TRIS AMINO is used for a number of purposes in its pure form, it is an acidimetric standard the USP grade can be utilized intraveneously for therapeutic control of blood acidosis TRIS AMINO also is useful in genetic engineering as a buffering agent for enzyme systems, industrial protein purification, and electrophoresis. AMP has found use as a reagent in enzyme-linked immunoassays. The primary appHcation is for alkaline phosphatase assays. 

The dehydrogenation of the mixture of m- and -ethyltoluenes is similar to that of ethylbenzene, but more dilution steam is required to prevent rapid coking on the catalyst. The recovery and purification of vinyltoluene monomer is considerably more difficult than for styrene owing to the high boiling point and high rate of thermal polymerization of the former and the complexity of the reactor effluent, which contains a large number of by-products. Pressures as low as 2.7 kPa (20 mm Hg) are used to keep distillation temperatures low even in the presence of polymerization inhibitor. The finished vinyltoluene monomer typically has an assay of 99.6%. 

Disodium 4-nitrophenylphosphate (6H2O) [4264-83-9] M 371.1 Dissolve in hot aqueous MeOH, filter and ppte by adding Me2CO. Wash the solid with Me2CO and repeat the purification. Aq MeOH and Et20 can also be used as solvents. The white fibrous crystals contain less than 1% of free 4-nitrophenol [assay J Biol Chem 167 57 1947]. 

Defects in arc-grown nanotubes place limitations on their utility. Since defects appear to arise predominantly due to sintering of adjacent nanotubes in the high temperature of the arc, it seemed sensible to try to reduce the extent of sintering by cooling the cathode better[2]. The most vivid assay for the extent of sintering is the oxidative heat purification treatment of Ebbesen and coworkers[7], in which amorphous carbon and shorter nanoparticles are etched away before nanotubes are substantially shortened. Since, as we proposed, most of the nanoparticle impurities orig-... 

For purification the crude quaternary salt was dissolved in hot ethyl alcohol (2 ml/g) and warm dry acetone (8 ml/g) was stirred into the clear filtrate. On cooling, 387 g (78% recovery) of a pure white powder, MP 195°Cto 197°C, were obtained, in which the ionizable chlorine assayed at 99.7% of the theoretical value. 

Process validation should be extended to those steps determined to be critical to the quality and purity of the enantiopure drug. Establishing impurity profiles is an important aspect of process validation. One should consider chemical purity, enantiomeric excess by quantitative assays for impurity profiles, physical characteristics such as particle size, polymorphic forms, moisture and solvent content, and homogeneity. In principle, the SMB process validation should provide conclusive evidence that the levels of contaminants (chemical impurities, enantioenrichment of unwanted enantiomer) is reduced as processing proceeds during the purification process. 

Benzothiadiazole 1,1-dioxide can be conveniently assayed and characterized without isolation by forming its adduct with cyclopentadiene.5 The following procedure illustrates characterization, for assay the same procedure can be applied to an aliquot, with all amounts scaled down in proportion. The dried ether extract of 1,2,3-benzothiadiazole 1,1-dioxide prepared from 1.43 g (0.0080 mole) of sodium 2-aminobenzene-sulfinate is concentrated to about 20 ml at 0°, and 20 ml. of acetonitrile at —20° is added. Twenty milliliters of cold, freshly prepared cyclopentadiene6 is added The mixture is kept overnight at —10° to 0°. Solvent and excess cyclopentadiene are removed by evaporation at 0° under reduced pressure to leave 1.20-1.28 g. (64-68% based on sodium 2-aminobenzenesulfinate) of crude 1-1 adduct, mp. 87° (dec.). For purification it is dissolved in 20 ml. of methylene chloride, 70 ml. of ether is added, and the solution is kept at —70°. Adduct decomposing at 90° crystallizes recovery is about 75%. From pure, crystalline 1, 2, 3-benzothiadiazole 1,1-dioxide the yield of adduct is 92-98%. 

Bacterial bioluminescence, 30-46 factors required, 31 general scheme, 32 in vivo luminescence, 41 luminescence reaction, 37, 38 Bacterial luciferase, 33-35, 343 assay, 39 cloning, 34 crystal structure, 34 extraction and purification, 34 inactivation, 34, 35 molecular weight, 34 properties, 34 storage, 35 subunits, 34... 

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