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Protein kinase assay

Buffer A Abl, Akt, Aurora, CDKs, CHK, cKit, cSrc, EGFR, GSK, JAK, PAK, PDGFR, PKA, Pyk2, SGK [Pg.87]


Volume 200. Protein Phosphorylation (Part A Protein Kinases Assays, Purification, Antibodies, Functional Analysis, Cloning, and Expression)... [Pg.24]

Immunoprecipitation for protein kinase assays using peptide substrates... [Pg.166]

Figure 6.34 Elastomer-based microwell protein kinase assay array. (From Zhu, H. et al.. Nature Genetics, 26, 283-289, 2000. With permission.)... Figure 6.34 Elastomer-based microwell protein kinase assay array. (From Zhu, H. et al.. Nature Genetics, 26, 283-289, 2000. With permission.)...
Koresawa, M. and T. Okabe. 2004. High-throughput screening with quantitation of ATP consumption a universal non-radioisotope, homogeneous assay for protein kinase. Assay Drug Dev. Technol. 2, 153-160. [Pg.23]

Assay of cyclic nucleotides by enzymatic cycling techniques Protein binding assay Protein kinase assay Radioimmunoassay... [Pg.293]

A CZE procedure for detection and assay of protein kinase and phosphatase activities in complex biological mixtures has been developed by Dawson et al. Thephosphorylated and dephosphorylated forms of several peptides were resolved using an uncoated capillary and a 150 mM phosphoric acid buffer at pH 2.0 or pH 5.0. Furthermore, the CZE-based assay was capable of resolving a peptide phosphorylated on different sites and permitted the quantification of each peptide. Since this application, much research has been done on protein kinase assays based on CE. [Pg.716]

Moon, J. H. MacLean, R McDaniel, W. Hancock, L. F. Conjugated polymer nanoparticles for biochemical protein kinase assay. Chem. Commun 2007,46, 4910-4912. [Pg.464]

Biochemical Applications. Cellulose phosphate paper (P81), also called phosphocellulose paper, has been used for enzyme assay applications (76). As with many other applications of cellulose phosphate, the ability of the material to act as an ion exchanger is the key property that leads to its use in enzyme assays. Cellulose phosphate paper quantitatively binds small peptides that contain at least two basic residues and a free amino terminus. This allows for protein kinase assays to be performed and P-labeled cofactors [y- P]ATP and nonpeptidic byproducts [ P]Pi to be washed away (77). [Pg.1092]


See other pages where Protein kinase assay is mentioned: [Pg.147]    [Pg.86]    [Pg.1200]    [Pg.1200]    [Pg.103]    [Pg.197]    [Pg.698]    [Pg.318]    [Pg.530]    [Pg.168]    [Pg.26]    [Pg.36]    [Pg.458]    [Pg.197]    [Pg.3098]    [Pg.175]   
See also in sourсe #XX -- [ Pg.2 ]

See also in sourсe #XX -- [ Pg.320 ]




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Proteins assay

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