Asperulae, Meliloti herba Toncae semen. .......... Fig

A Methanolic extracts of Meliloti herba (1), Asperulae herba (2) and Toncae semen (3) contain the unsubstitllted coiimarin (T2), which is seen as a prominent quenching xone at R, 0.6 (UV-254 nm). 

B In UV-36.5 nm, the un.sub,stituted coumarin, in contrast to coumarins with -OH, -OCH,, sLib,stituents or fiirano- or pyrano-coumarins, shows a typical green-blue fluorescence only after treatment with KOH reagent. Scopoletin (Tl) and umbelliferone (T3) are present in low concentrations only, seen as blue fluorescent zones at Rj 0.25 and 0.4, rc.spcctively (1-3). 

B Treatment with the NP/PEG reagent reveals bright orange-red, yellow-green and blue fluorescent zones in UV-365 nm. 

Solvent system Fig. 3 toluene-ether ] l/saturated with 10% acetic add) 

I Abrotani herba 2 fabiani herba (= Pichi-Pichi) 

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