Herba Asperulae

Asperulae herba (ethyl acetate extract/chlorophyll free, 20. il)... 

Fig, A Methanolic extracts of Meliloti herba (1), Asperulae herba (2) and Toncae semen (3) contain the unsubstitllted coiimarin (T2), which is seen as a prominent quenching xone at R, 0.6 (UV-254 nm). 

Fig. 2A Meliloti herba (1,1a) and Asperulae herba (2,2a). show prominent quenching zones of flavonoid glycosides and caffeic acid derivatives in different patterns and amounts. The coumarins (T1-T3), flavonoid aglycones and caffeic add migrate almost up to the solvent front, Flavonoid glycosides and chlorogenic acid arc found from R, — 0.05 to 0.5. Extracts of Meliloti herba (1,1a) show mainly one prominent zone at R, -- 0.5, while Asperulae herba (2,2a) has four almost equally concentrated zones in the R, range 0.25-0.5.

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