Herba Abrotani

I Abrotani herba 2 fabiani herba (= Pichi-Pichi)... 

The metlianolic extracts of Abrotani herba (1) and Fabiani herba (2) are characterized by the prominent blue fluorescent coumarin aglycoiie zone at R, 0.95 (scopoletin, isofraxidin and umbelliferone). They are differentiated by the violet-blue fluoresceiit isofraxidin-7-0 and scopoletin-7-O-glucosides in the range of0.4-0.45 and an additional zone at R 0.7 in sample 1 and the scopoletin-7-O-priinveroside at Rf 0.15 in extract 2. 

The coumarin-7-O-glucosides of Abrotani herba (1) are seen with KOH reagent as two fluorescent zones at R, 0.4-0.45. The aglycones at R, --- 0.95 become bright blue. 

Treatment with the NP-PfiG reagent shows in Abrotani herba (1) abroad band of intense bluish-white fluorescent zones of coumarins and phenolcarboxylic adds in the R, range 0.35 up to the solvent front, which overlay the orange fluorescent flavonoid glycosides (Rf 0.4 (rutin), Rf 0.6-0.65 e.g. hyperosid, isotjuercitrin) and the couraarin-7-0-glucosides. 

The caffeic acid derivatives with chlorogenic add at R, 0.45 (Tl) and isochlorogenic acids at RfO. 7-0.8 are more concentrated in Abrotani herba (1) than in Fabiani herba (2). The later shows rutin at Rf — 0.4 as a prominent orange zone and tlie violet-blue Zone of scopoletin-7-O-primveroside at Rf 0.15. Blue coumarin and orange fluorescent flavonoid aglycones move with the solvent front,... 

---