Asparagine/aspartate

Alanine Arginine Asparagine Aspartic Acid Cysteine... 

It is noteworthy that there is another limiting factor in the choice of amino acid types at the junction sites which affect the enzymatic process of the intein. For example, in the case of SceVMA (also called PI-Seel) from the IMPACT system, proline, cysteine, asparagine, aspartic acid, and arginine cannot be at the C-terminus of the N-terminal target protein just before the intein sequence. The presence of these residues at this position would either slow down the N-S acyl shift dramatically or lead to immediate hydrolysis of the product from the N-S acyl shift [66]. The compatibility of amino acid types at the proximal sites depends on the specific inteins and needs to be carefully considered during the design of the required expression vectors. The specific amino acid requirements at a particular splicing site depends on the specific intein used and is thus a crucial point in this approach. 

L-Amino acids, 2.5 X 10+6 molar solution of alanine, arginine, asparagine, aspartic acid, cysteine, cystine, glutamine, glutamic acid, glycine, histidine, hydroxyproline, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine. 

Alanine Arginine Asparagine Aspartate Cysteine Glutamate Glutamine Glycine Histidine Proline Serine Tyrosine ... 

H2N0CCH2CH(NH2)C02H H02CCH2CH(NH2)C02 h asparagine aspartic acid... 

The most popular test for the presence or absence of free amino groups is the Kaiser test.10 The test is simple and quick however it should be noted that some deprotected amino acids do not show the expected dark blue color typical of free primary amino groups (e.g., serine, asparagine, aspartic acid).11 Furthermore, for secondary amines such as proline, the resin will turn brown instead of blue. For secondary amines and aromatic amines, the chloranil test is recommended.12 In this volume, Albericio s research... 

In this assay the asparagine, aspartate, glutamine, and glutamate are separated by reversed-phase HPLC (Cj8) using a mobile phase of 70% sodium acetate buffer (pH 5.9), and 30% methanol as shown in Figure 9.44. 

Imamura, T., Fujita, S., Ohita, Y., Hanada, M., and Yanase, T., Hemoglobin Yoshizuka (GIO (108)/3 Asparagine— Aspartic Acid) a new variant with a reduced oxygen affinity from a Japanese family. J. Clin. Invest. 48, 2341-2348 (1969). 

The 11 nonessential amino acids are listed as follows alanine, asparagine, aspartate, cysteine, glutamine, glutamate, glycine, hydroxyproline, proline, serine, and tyrosine. They are synthesized within the body, for instance, from essential amino acids. Interestingly, however, hydroxyproline is not one of the 20 common amino acids — the latter being the 20 as established by means of the genetic code. 

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