Asparaginase isolation

Photocontrolled affinity chromatography of asparaginase Isolation of testosterone-binding globulin from bovine serum by affinity chromatography... 

Using different strains of E coli, different isoenzymes of i-AS have been isolated [15]. The molecular weight was found to be 120 kDa with some isoenzymes having 240 kDa but lower specific enzyme activity. All asparaginases am obviously composed of four subunits whose molecular weight was initially... 

Roberts ), Holcenberg JS, Dolowy WC. Isolation, crystallization, and properties of Achro-mobacteriaceae glutaminase-asparaginase with antitumor activity./. Biol. Chem. 247(1) 84-90, 1972. 

An isolated case report su ests that vincristine neurotoxicity may possibly have been increased by subsequent asparaginase therapy. The UK manufacturer recommends that vincristine should be given 12 to 24 hours before asparaginase. Regimens including both drugs are commonly used in treating leukaemia. 

A glutaminase asparaginase enzyme isolated from Achromatobacteriaceae was administered to 9 patients suffering from acute leukaemia. Toxicity manifestations were nausea, vomiting and neutropenia in most of the patients drowsiness, depression of serum urate, bicarbonate, cholesterol, GOT, calcium and thrombocyte number, and an increase in serum phosphate concentration, in only one or two of them. Metabolic acidosis (blood pH 7.3), necessitating interruption of the treatment and reversed by intravenous sodium bicarbonate was observed in one patient (24 ). 

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