Glutaminase asparaginase

The heat stability of glutaminase-asparaginase modified with glu-taraldehyde-activated glycopeptides was slightly greater than that of the native enzyme, as was its susceptibility to trypsin.108 This modification decreased the pi by several pH units, and altered the association behavior the glycosylated enzyme had a much wider distribution of molecular weights as determined by sedimentation equilibrium. 

A. Abudiowni, F. F. Davis, and 1 Davis, immunosuppressive properties and circulating life of achromobacter glutaminase-asparaginase covalently attached to polyethylene glycol in man. Cancer Treat. Sep. 65 1077 (1981). 

In the case of unmodified Pseudomonas 7A Glutaminase-asparaginase (PGA), the plasma half-life in normal BDFl mice is 13 hours [22], but this is extended by PEG-ylation to approximately 5 days in mice, rats, and rabbits (Figs. 6.5-6.8). Thus, the PEGylated drug requires much less-fre-... 

Roberts ), Holcenberg JS, Dolowy WC. Isolation, crystallization, and properties of Achro-mobacteriaceae glutaminase-asparaginase with antitumor activity./. Biol. Chem. 247(1) 84-90, 1972. 

Roberts J. Purification and properties of a highly potent antitumor glutaminase-asparaginase from Pseudomonas 7A./. Biol. Chem. 251 2119-2123, 1976. 

Roberts J, McGregor WG. Inhibition of mouse retroviral disease by bioactive glutaminase asparaginase./. Gen. Virol. 72 299-305, 1991. 

Prous Integrity database 453 Pseudomonas 7A glutaminase-asparaginase see PGA... 

Conjugates between polysaccharides (e.g. dextrans, starch, and dextrin) and proteins (e.g. lysozyme, chymotrypsin, j8-glucosidase, casein, and serum albumin) have been prepared with the aid of cyanogen bromide. Loss of enzymic activity was offset by increases in the thermal stability and the resistance to proteolytic digestion. Glutaminase-asparaginase from Acinetobacter has been... 

A glutaminase asparaginase enzyme isolated from Achromatobacteriaceae was administered to 9 patients suffering from acute leukaemia. Toxicity manifestations were nausea, vomiting and neutropenia in most of the patients drowsiness, depression of serum urate, bicarbonate, cholesterol, GOT, calcium and thrombocyte number, and an increase in serum phosphate concentration, in only one or two of them. Metabolic acidosis (blood pH 7.3), necessitating interruption of the treatment and reversed by intravenous sodium bicarbonate was observed in one patient (24 ). 

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