Artificial extracellular matrix

Fig. 15 Amino acid sequences of artificial extracellular matrix (aECM) proteins. Each protein contains a TV tag, a histidine tag, a cleavage site, and elastin-like domains with lysine residues for crosslinking. The RGD cell-binding domain is found in aECM 1, whereas aECM 3 contains the CS5 cell-binding domain. aECM 2 and aECM 4 are the negative controls with scrambled binding domains for aECM 1 and aECM 3, respectively. Reprinted from [121] with permission from American Chemical Society, copyright 2004...

Panitch, A., Yamaoka, T., Fournier, M. J., Mason, T. L., and Tirrell, D. A. (1999). Design and biosynthesis of elastin-like artificial extracellular matrix proteins containing periodically spaced fibronectin CS5 domains. Macromolecules 32, 1701-1703. 

Ohya S, Nakayama Y, Matsuda T. Thermoresponsive artificial extracellular matrix for tissue engineering hyaluronic acid bioconjugated with poly(N-iso-propylacrylamide) grafts. Biomacromolecules 2001 2 856-863. 

N. Chen, Z. Zhang, B. Soontornworajit, J. Zhou, Y. Wang, Celladhesion on an artificial extracellular matrix using aptamer-functionalized PEG hydrogels, Biomaterials 33 (5) (2012) 1353-1362. 

Schnabelrauch, M., Scharnweber, D., and Schiller, J. 2013. Sulfated glycosaminogly-cans as promising artificial extracellular matrix components to improve the regeneration of tissues, Curr. Med. Chem., 20 2501-2523. 

Liu JC, Heilshom SC, Tirrell DA (2004) Comparative cell response to artificial extracellular matrix proteins containing the RGD and CS5 cell-binding domains. Biomacromolecules 5 497-504... 

Nowatzki PJ, Tirrell DA (2004) Physical properties of artificial extracellular matrix protein films prepared by isocyanate crosslinking. Biomaterials 25 1261-1267... 

A further major field of investigation for bioerodihle polymers is their use as scaffolds for tissue engineering. Tissue engineering is the use of a bioerodible polymer as an artificial extracellular matrix, supporting cell growth and organisation and will he discussed in Chapter 4, as it represents a major focus of polyphosphazene research. 

Kasoju, N., Bora, U., 2012. SiUt fibroin based biomimetic artificial extracellular matrix for hepatic tissue engineering applications. Biomed. Mater. 7, 045004 (1299). 

Morikawa, N., Matsuda, T. (2002). Thermoresponsive artificial extracellular matrix A-isopropylacrylamide-graft-copolymerized gelatin. Journal of Biomateriai Science Polymer Edition, 13, 167-183. 

Heilshorn, S.C. et aL, Endothelial cell adhesion to the fibronectin CS5 domain in artificial extracellular matrix proteins. Biomaterials 24,4245-52, 2003. 

Ohya, S., Matsuda, T., Poly(N -Isopropylacrylamide) (PNIPAM)-Grafted Gelatin as Thermoresponsive Three-Dimensional Artificial Extracellular Matrix Molecular and Formulation Parameters vs. Cell Proliferation Potential, Riomater. Sd. Polvm. Fdn. 2005, 16, 809-827. 

Ohya, S. and Matsuda, T. (2005). Poly(N-isopropylacrylamide) (PNIPAM)-grafted gelatin as thermoresponsive three-dimensional artificial extracellular matrix molecular and formulation parameters vs. cell proliferation potential. Journal of Biomaterials Science-Polymer Edition, 16,809-827. 

Tissue engineering is a medical engineering technology that offers a promising new approach to create biological alternatives for regenerating different tissues. An artificial extracellular matrix in which cells can proliferate and differentiate with subsequent new tissue generation is critical in tissue... 

M. Schnabelrauch, D. Scharnweber and J. Schiller, Sulfated Glycosami-noglycans as Promising Artificial Extracellular Matrix Components to Improve the Regeneration of Tissues, Curr. Med. Chem., 2013, 20, 2501. 

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