Artemia hatching

As mentioned earlier, larval feed companies, for these reasons, have developed specialized products which contain bacteriostatic agents to keep bacterial loads within acceptable levels. These include specially treated cysts, enrichment products with bacteriostatic properties, or separate formulations which can be added to the Artemia hatching or enrichment medium. Also at the research level, several attempts have been made to disinfect Artemia nauplii (e.g. Gatesoupe, 2002 Tolomei et al., 2004 Gimenez et al., 2006). These include treatment of either cysts or the hatched nauplii with biocides (e.g. formaldehyde), UV, ozone or peroxide-based products. Apart from the fact that these treatments might cause considerable mortality to the nauplii or reduce their vigour, they might also pose potential risk to the predator larvae they are fed to, because of residues or toxic by-products produced due to these treatments. 

Brine shrimp, Artemia sp. 14-1360 Egg hatching significantly reduced in 28... 

We also examined the effect of petuniolide C upon the brine shrimp, Artemia salina, in order to test the response of another class of arthropod toward this chemical. This crustacean has been suggested as a general experimental subject in toxicity determination of chemical substances toward invertebrates, and for the indication of cytotoxicity (21, 22). Solutions for the test were prepared by adding stock solutions of XVIII in ethanol to containers of artificial salt water (1% EtOH final concentration) with sonic agitation. Even with added ethanol, the solubility limit of the steroid was about 1.0 ppm. Newly hatched brine shrimp were added to these solutions, and controls were run using salt water and salt water containing 1% ethanol. After 24 hr., no mortality was observed for the EtOH controls, and no toxic effect was seen for petuniolide C at 1.0 ppm. 

BRisKi E, VAN STAPPEN G, BOSSIER p and SORGELOOS p (2008) Laboratory prodnction of early hatching Artemia sp. cysts by selection. Aquaculture 282 19-25. 

LAVENS p and sorgeloos p (1987) The cryptobiotic state of Artemia cysts, its diapanse deactivation and hatching, in Sorgeloos P, Bengtson D A, Decleir W and Jaspers E (eds), Artemia Research and its Applications. Vol. 3. Wetteren Universa Press, 27-63. 

VANHAECKE p and SORGELOOS p (1989) International Study on Artemia. XLVII. The effect of temperature on cyst hatching, larval survival and biomass production for different geographical strains of brine shrimp Artemia spp. Annals of the Royal Zoological Society of Belgium 119(1) 7-23. 

Fig. 12 Increase in dry weight (mg) of Octopus vulgaris paralarvae from hatching to day 45 using large Artemia and crab zoeae, (from Iglesias et ai, 2004). Reproduced with permission from Springer...

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