ARM complexes for In vitro display and evolution of antibody combining sites

ARM complexes for in vitro display and evolution of antibody combining sites 

Laboratory of Molecular Recognition, The Babraham Institute, Babraham, Cambridge CB2 4AT, U.K. 

Rgure 1 Ribosome display cycle for antibody selection (ARM display). ARM antibody-ribosome-mRNA complex. 

Initially, display of a peptide library on prokaryotic polysomes in the i . coK S30 system was described (6, 7). To produce a population of stalled polysomes, agents such as rifampicin or chloramphenicol, which block prokaryotic translation, were used. (The term stalled implies that the process of translation has been interrupted such that the ribosome. niRNA, and nascent protein remain associated.) The main example was of screening a large peptide library with 10 members and selection of epitopes by a specific antibody. Peptide libraries have also been expressed and selected in the wheat germ system (8). 

We have developed an alternative method of ribosome display based on 

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