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ARM display

Figure 1 Ribosome display cycle for antibody selection (ARM display). ARM = antibody ribosome-mRNA complex. Figure 1 Ribosome display cycle for antibody selection (ARM display). ARM = antibody ribosome-mRNA complex.
Primer design and single chain antibody (Vh/19 construction for ARM display... [Pg.93]

No. K2003(M)l/40/J10). The ARM method can be used for screening and identification of functional clones prior to E. cdi expression analysis. Plasnrid DNA is prepared and amplified by PCR for ARM display. In order to quantify recovery and rule out differences caused by the variable efficiency of RT-PCR, an internal control in the form of an mRNA carrying the same flanking sequences but of... [Pg.103]

Screening of clones by ARM display using an Internal standard In RT-PCR... [Pg.104]

Carry out ARM display on individual clones and isolate complexes on beads (Protocols 2-4). [Pg.104]

Consecutive cydes of ARM display enabled selection from libraries in which the binding fragment was present at a lower frequency. Thus a 1 10 DB3 ... [Pg.106]

Figure 5 Enrichment of DB3 from a 1 10 (DB3 DB3 ) library by repeated ARM display cycles. Selection was with pro sterone-llot-BSA coupled beads. Track 1 1 kb DMA marker. Tracks 2, 3, 4 RT-PCR after first, second, and third cycles respectively. Figure 5 Enrichment of DB3 from a 1 10 (DB3 DB3 ) library by repeated ARM display cycles. Selection was with pro sterone-llot-BSA coupled beads. Track 1 1 kb DMA marker. Tracks 2, 3, 4 RT-PCR after first, second, and third cycles respectively.
ARM display is the application of eukaiyotic ribosome display to selection and evolution of antibody combining sites. It potentially displays very large libraries... [Pg.107]

See other pages where ARM display is mentioned: [Pg.93]    [Pg.98]    [Pg.104]    [Pg.104]    [Pg.105]    [Pg.108]    [Pg.108]    [Pg.484]    [Pg.490]    [Pg.505]    [Pg.505]    [Pg.93]    [Pg.98]    [Pg.103]    [Pg.104]    [Pg.104]    [Pg.105]    [Pg.108]    [Pg.108]    [Pg.485]   
See also in sourсe #XX -- [ Pg.91 , Pg.92 , Pg.93 , Pg.94 , Pg.95 , Pg.96 , Pg.97 , Pg.98 , Pg.99 , Pg.100 , Pg.101 , Pg.102 , Pg.103 , Pg.104 , Pg.105 , Pg.106 , Pg.107 , Pg.108 ]

See also in sourсe #XX -- [ Pg.91 , Pg.92 , Pg.93 , Pg.94 , Pg.95 , Pg.96 , Pg.97 , Pg.98 , Pg.99 , Pg.100 , Pg.101 , Pg.102 , Pg.103 , Pg.104 , Pg.105 , Pg.106 , Pg.107 , Pg.108 ]



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ARM complexes for In vitro display and evolution of antibody combining sites

Analysis of clones encoding antibodies by ARM display

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