Are given in detail on page

Names in small capital letters refer to the titles of individual preparations. A number in ordinary bold-face type denotes the volume. A number in bold-face italics refers to a page which gives preparative directions for substances formed either as principal products or as by-products numbers in ordinary type indicate pages on which a compound or a subject is mentioned in connection with other preparations. For example. Acetone cyanohydrin, SO, 42, 43, indicates that acetone cyanohydrin is mentioned on page 42, and that directions for its preparation are given in detail on page 43, of Volume 20. 

Throughout, several sources of data are repeatedly cited as refs, (a, b, c etc.). Details of these are given in the Bibliography on page 224. 

This chapter will describe in detail the procedure for Western blotting of polypeptides and proteins separated on a denaturing polyacrylamide gel system. This procedure is used routinely in our laboratory for the analysis of polypeptides from a variety of subcellular fractions of whole tissue and cell lines, and has evolved over a number of years in the hands of several people. Many different immunoblotting procedures are currendy available details of variadons from the I-protein-A method described here are given in the Notes secdon, as are brief amendments covering electrotransfer from two-dimensional and isoelectric focusing systems. A detailed descripdon of sodium dodecyl sulfate polyacrylamide electrophoresis (SDS-PAGE) is not appropriate for this chapter, and the reader is referred to vol. 1, Chapter 6, and refs. 9-14. For details of the producdon of polyclonal and monoclonal andsera, Chapters 1-6 in this vol. 

The enzyme urease, which catalyzes the hydrolysis of urea, is widely used to determine urea in blood. Details of this application are given in Feature 29-3 on page 901. The Michaelis constant for urease at room temperature is 2.0 mM, and k2 = 2.5 X 10 s at pH 7.5. (a) Calculate the initial rate of the reaction when the urea concentration is 0.030 niM and the urease concentration is 5.0 p,M, and (b) find v, ax-... 

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