Applications of PCR to agricultural biotechnology

The PCR technique is very useful during all stages of the research and development of biotech crops. PCR analysis is used for gene discovery, event selection, screening, transformant identification, line selection and plant breeding. Quantitative real-time PCR is used to determine the number of transgene copies inserted in experimental 

PCR is used to support regulatory submissions. For example, a petition for nonregu-lated stams for a biotech crop must contain the following information  

PCR analysis is one of the techniques used to generate data for the genetic analysis requirement. 

These PCR laboratories often offer GMO screening, specific tests for certain commercial GMOs and real-time quantitative testing. The different approaches vary widely in cost and the choice would depend on the testing objective. 

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