Applications for Reversed-Phase Chromatography

Reversed-phase chromatography is the most popular mode for the separation of low molecular weight ( 3000), neutral species that are soluble in water or other polar solvents. It is widely used in the pharmaceutical industry for separation of species such as steroids, vitamins, and /3-blockers. It is also used in other areas for example, in clinical laboratories for analysis of catecholamines, in the chemical industry for analysis of polymer additives, in the environmental arena for analysis of pesticides and herbicides, and in the food and beverage industry for analysis of carbohydrates, sweeteners, and food additives. 

Mutant form of rt-PA with Glutamic Acid at Position 275 

Chapter 2 Separations in High-Performance Liquid Chromatography 

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