Apparent enzyme activities, adsorbed layer

Apparent Enzyme Activity and Km Value of Adsorbed Layers and Enzyme Membranes... 

Table 14-6. Apparent enzyme activities and Kf values of adsorbed layers and enzyme membranes.

In Table 5 the apparent activities of enzymes entrapped in or covalently fixed to membranes are compared to those of enzymes directly adsorbed or fixed to electrode surfaces. It may be concluded that different immobilization procedures lead to approximately identical apparent activities. The advantage of direct attachment to the transducer surface is the low diffusion resistance of the monomolecular enzyme layer. On the other hand, enzyme membranes are more stable because of their inherent enzyme excess. 

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